背根神经节核苷酸寡聚化域样受体3在大鼠持续性术后痛中的作用  

Role of Nod-like receptor family pyrin domain-containing 3 in dorsal root ganglion in persistent postoperative pain in rats

作　　者：李晨 陈晓烨 陈晨[1] 杨林译[1] 沈江[1] 李敏[1] Li Chen;Chen Xiaoye;Chen Chen;Yang Linyi;Shen Jiang;Li Min(Department of Anesthesiology,The Third Hospital of Soochow University,Changzhou 213003,China)

机构地区：[1]苏州大学附属第三医院麻醉科,常州213003

出　　处：《中华麻醉学杂志》2021年第10期1234-1237,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价背根神经节核苷酸寡聚化域样受体3(NLRP3)在大鼠持续性术后痛中的作用。方法取鞘内置管成功的雄性SD大鼠48只,体重200~250 g,2~3月龄,采用随机数字表法分为4组(n=12):假手术组(S组)、持续性术后痛组(P组)、持续性术后痛+NLRP3-siRNA组(P+siRNA组)和持续性术后痛+NLRP3-scrRNA组(P+scrRNA组)。通过皮肤/肌肉切口和牵拉法建立持续性术后痛模型。于术前3 d时,S组和P组鞘内注射生理盐水10μl,P+siRNA组和P+scrRNA组分别鞘内注射NLRP3-siRNA 10μl和NLRP3-scrRNA 10μl。分别于术前1 d(T0)和术后1、5、10、15、20 d(T1~5)时测定机械缩足反应阈(MWT);于T3时每组随机处死6只大鼠,取术侧L4,5节段背根神经节,采用Western blot法检测NLRP3和caspase-1的表达水平,RT-PCR法检测NLRP3 mRNA表达水平,ELISA法测定IL-1β含量。结果与S组比较,P组T2~5时MWT降低,背根神经节NLRP3及其mRNA和caspase-1的表达上调,IL-1β含量升高(P<0.05);与P组比较,P+siRNA组T2~5时MWT升高,背根神经节NLRP3及其mRNA和caspase-1的表达下调,IL-1β含量降低(P<0.05),P+scrRNA组上述指标差异无统计学意义(P>0.05)。结论背根神经节NLRP3参与了大鼠持续性术后痛的发生发展,机制可能与形成NLRP3炎症小体诱发外周神经炎症反应有关。Objective To evaluate the role of Nod-like receptor family pyrin domain-containing 3(NLRP3)in the dorsal root ganglion(DRG)in persistent postoperative pain in rats.Methods Forty-eight male Sprague-Dawley rats,in which IT catheters were successfully implanted,weighing 200-250 g,aged 2-3 months,were divided into 4 groups(n=12 each)using a random number table method:sham operation group(S group),persistent postoperative pain group(P group),persistent postoperative pain+NLRP3-siRNA group(P+siRNA group)and persistent postoperative pain+NLRP3-scrRNA group(P+scrRNA group).A persistent postoperative pain model was established by skin/muscle incision and retraction(SMIR)in anesthetized animals.Normal saline 10μl were intrathecally injected in S group and P group,NLRP3-siRNA 10μl and NLRP3-scrRNA 10μl were intrathecally injected in CP+siRNA group and CP+scrRNA group,respectively,at 3 days before operation.The mechanical paw withdrawal threshold(MWT)was measured at 1 day before operation(T0)and 1,5,10,15 and 20 days after operation(T1-5).Six rats in each group were randomly selected and sacrificed at T3,and the L4-5 DRGs on the operated side were harvested for determination of the expression of NLRP3 and caspase-1(by Western blot),NLRP3 mRNA expression(by real-time polymerase chain reaction)and content of interleukin-1beta(IL-1β)(by enzyme-linked immunosorbent assay).Results Compared with group S,the MWT was significantly decreased at T2-5,the expression of NLRP3 protein and mRNA in DRGs was up-regulated,and the content of IL-1βwas increased in group P(P<0.05).Compared with group P,the MWT was significantly increased at T2-5,the expression of NLRP3 protein and mRNA and caspase-1 was down-regulated,and the content IL-1βwas decreased in group P+siRNA(P<0.05),and no significant change was found in the parameters mentioned above in group P+scrRNA(P>0.05).Conclusion NLRP3 in DRGs is involved in the development of persistent postoperative pain,and the mechanism may be related to the development of NLPR3 inflammasomes which

关 键 词：NLR家族 热蛋白结构域包含蛋白3 神经节 脊 疼痛 手术后 

分 类 号：R619[医药卫生—外科学]