基于Hadamard优化矩阵的多分辨显微关联成像研究  

Study of Multi-Resolution Microscopic Correlation Imaging Based on Optimized Hadamard Matrix

作　　者：冯悦姝 周成 刘轩[3] 刘小涵 宋立军 Feng Yueshu;Zhou Cheng;Liu Xuan;Liu Xiaohan;Song Lijun(Institute for Interdisciplinary Quantum Information Technology,Jilin Engineering Normal University,Changchun,Jilin 130052,China;Jilin Engineering Laboratory for Quantum Information Technology,Changchun,Jilin 130052,China;School of Electronic Information Engineering,Changchun University of Science and Technology,Changchun,Jilin 130022,China)

机构地区：[1]吉林工程技术师范学院量子信息技术交叉学科研究院,吉林长春130052 [2]吉林省量子信息技术工程实验室,吉林长春130052 [3]长春理工大学电子信息工程学院,吉林长春130022

出　　处：《光学学报》2021年第21期116-124,共9页Acta Optica Sinica

基　　金：吉林省科技发展计划项目(20200404141YY);吉林省教育厅“十三五”科学研究规划项目(JJKH20200171KJ)。

摘　　要：为满足显微成像领域的多样化需求,解决实际应用中成像质量与成像时间之间的矛盾,提出一种基于数字微镜器件的多分辨显微关联成像方法。该方法利用LED光源作为背景照射光源,对科研级荧光正置显微镜原光路改装设计为关联成像光路,采用多分辨Hadamard优化矩阵作为数字微镜器件的预置图样,实现了生物组织样品的连续多分辨成像。实验结果表明,多分辨显微关联成像系统的分辨率可达218 nm,单组测量后可同时输出8组不同分辨率图像,能够根据实际应用中不同图像质量需求选择不同的分辨率,减少成像时间和存储空间,极大地提高了显微成像的灵活性。这种新型多分辨显微关联成像方法可以扩展至细胞筛选、细胞实时成像等领域,对推动关联成像在细胞和生物组织显微成像领域的应用具有重要意义。A multi-resolution microscopic correlation imaging method based on a digital micromirror device was proposed to meet the diverse needs of microscopic imaging and resolve the contradiction between imaging quality and imaging time in practical applications.In this method,an LED was used as the background illumination source,and the original optical path of a research-grade upright fluorescence microscope was modified to be an optical path of correlation imaging.The multi-resolution optimized Hadamard matrix was adopted as the preset pattern of the digital micromirror device.Continuous multi-resolution microscopic imaging of biological tissue samples was achieved by this system.The experimental results demonstrate that the resolution of the multi-resolution microscopic correlation imaging system reaches 218 nm.Eight groups of images in different resolutions can be output simultaneously after a single measurement.Therefore,different resolutions can be selected according to particular image quality requirements in practical applications.By reducing the imaging time and storage space needed,this system greatly improves the flexibility of microscopic imaging.The proposed multi-resolution microscopic correlation imaging method can be extended to fields such as cell screening and real-time cell imaging.It is expected to promote the application of correlation imaging in microscopic imaging of cells and biological tissues.

关 键 词：成像系统 显微成像 关联成像 数字微镜器件 Hadamard优化矩阵 多分辨成像 

分 类 号：O438[机械工程—光学工程]