蠋蝽病毒Arma chinensis virus-1基因组克隆及序列分析  

Cloning and sequence analysis of Arma chinensis virus-1 genome

作　　者：孙梅雪 张长华 高强 李炜 贾芳曌 徐蓬军[1] 任广伟[1] SUN Meixue;ZHANG Changhua;GAO Qiang;LI Wei;JIA Fangzhao;XU Pengjun*;REN Guangwei(Tobacco Research Institute of CAAS,Qingdao 266101,Shandong,China;Zunyi Branch of Guizhou Provincial Tobacco Company,Zunyi 563000,Guizhou,China;Yishui Branch of Shandong Linyi Tobacco Co.,Ltd.,Yishui 276400,Shandong,China)

机构地区：[1]中国农业科学院烟草研究所,山东省青岛市266101 [2]贵州省烟草公司遵义市公司,贵州省遵义市563000 [3]山东临沂烟草有限公司沂水分公司,山东省沂水县276400

出　　处：《烟草科技》2021年第12期9-16,共8页Tobacco Science & Technology

基　　金：中国烟草总公司贵州省公司项目“规模化繁育中蠋蝽种群染病规律及其防控技术研究”(201938)和中国烟草总公司山东省公司项目“沂蒙丘陵生态区绿色防控示范区建设”(201908)。

摘　　要：为明确天敌昆虫蠋蝽病原物的基因组结构,利用高通量测序技术,在蠋蝽体内发现了一种新的正义单链RNA病毒,并将其命名为Arma chinensis virus-1(AcV-1)。利用PCR和RACE扩增获得了含有完整读码框的AcV-1基因组序列,除Poly(A)外,其基因组长度为9192 nt,包含2个开放阅读框(Open reading frames,ORFs)。ORF1长5316 nt,编码一个含1771个氨基酸的非结构蛋白,包含3个保守结构域,分别是依赖RNA的RNA聚合酶、蛋白酶和RNA解旋酶;ORF2长2529 nt,编码一个含842个氨基酸的结构蛋白。AcV-1基因组序列与蟋蟀麻痹病毒(Cricket paralysis virus,CrPV,GenBank:KP974707.1)的相似性(89.40%)最高。根据序列同源性、基因组结构和聚类分析表明,AcV-1是蟋蟀麻痹病毒属(Cripavirus)的一个新成员。AcV-1在供试蠋蝽样本中侵染率为37.67%。In order to clarify the genome structure of pathogen for natural enemy insect Arma chinensis,a novel single-stranded RNA virus was detected in Arma chinensis through high-throughput sequencing and was named as Arma chinensis virus-1(AcV-1).The genome sequence of AcV-1 with two complete open reading frames(ORFs)amplified by PCR and RACE was 9192 nt in length excluding the poly(A)tail.The predicted ORF1 was 5316 nt in length,encoded a non-structural protein consisting of 1771 amino acids and contained 3 conserved domains including RNA-dependent RNA polymerase,peptidase and RNA helicase.The predicted ORF2 was 2529 nt in length and encoded structural proteins consisting of 842 amino acids.The similarity of genome sequence between AcV-1 and Cricket paralysis virus(GenBank:KP974707.1)was the highest(89.40%).Sequence homology,genome structure and phylogenetic analysis indicated that AcV-1 was a novel member of genus Cripavirus.The infection rate of AcV-1 in tested samples of Arma chinensis was 37.67%.

关 键 词：蠋蝽 Arma chinensis virus-1 基因组结构 系统进化 

分 类 号：TS413[农业科学—烟草工业]