耐重金属肠杆菌GL-2鉴定及对小麦促生作用的研究  

Identification on heavy metal-resistant Enterobacter ludwigii GL-2 and effects on growth of wheat

作　　者：蔡高磊 周炀 赵昌松 彭宣和 张振 赵建宁 CAI Gao-lei;ZHOU Yang;ZHAO Chang-song;PENG Xuan-he;ZHANG zhen;ZHAO Jian-ning(Key Laboratory for Environmental Factors Control of Agro-product Quality Safety,Ministry of Agriculture and Rural Affaris,Tianjin 300191,China;Danjiangkou Reservoir Shiyan Ecological Agriculture Institute,Shiyan 442000,Hubei,China;Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin 300191,China)

机构地区：[1]农业农村部农产品质量安全环境因子控制重点实验室,天津300191 [2]丹江口库区十堰生态农业研究院,湖北十堰442000 [3]农业农村部环境保护科研监测所,天津300191

出　　处：《湖北农业科学》2021年第23期44-48,共5页Hubei Agricultural Sciences

基　　金：农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放课题基金项目(18nybcdhj-6);中国农业科学院科技创新工程协同创新任务(CAAS-XTCX2016015)。

摘　　要：为分离出具有重金属抗性的植物根际促生菌,用添加了Cd的LB培养基和嗜铁素培养基筛选合适的菌株,用平板稀释法测定GL-2菌株的最低抑制浓度。在不同处理下的MS培养基中研究了GL-2菌株下Pb和Cd对小麦生长的影响。利用盆栽试验研究了GL-2对小麦生长的促进作用。结果表明,分子生物学和生理生化鉴定GL-2菌株为路氏肠杆菌(Enterobacter ludwigii);GL-2对重金属具有很强的耐受性,其中对Pb的最低抑制浓度高达650 mg/L;添加GL-2菌株的MS培养基可有效减轻重金属对小麦生长的影响;GL-2菌株显著增加了小麦根鲜重、分蘖数和根干重。In order to isolate the plant growth-promoting rhizobacteria(PGPR)that exhibit heavy metal resistance,the LB medium supplemented with Cd and the siderophores medium were used to screen suitable strains.Minimal inhibitory concentrations(MICs)of GL-2 strain was determined by plate dilution method.The passivation effects to Pb and Cd on the growth of wheat under GL-2 strain were added to MS medium in the different treatments.The promoting effect of GL-2 was studied on the growth of wheat by pot experiments.The results showed that the strain GL-2 belongs to Enterobacter ludwigii based on molecular biology and physiological and biochemical identification.GL-2 had a strong tolerance to heavy metals,and the minimum inhibitory concentration of Pb was 650 mg/L.The MS medium added to GL-2 strains could relieve heavy metal influence on wheat growth effectively.There was a significantly effect to increase the root fresh weight,tiller numbers and root dry weight of wheat within GL-2 strains.

关 键 词：路氏肠杆菌(Enterobacter ludwigii) 小麦 重金属 促生作用 

分 类 号：S512.1[农业科学—作物学]