PpPRR5基因在不同光合速率梨品种中的表达分析  

Expression analysis of PpPRR5 gene in pear genotypes with different degrees of photosynthetic rate

作　　者：刘政[1] 安琳 伍涛[1] 秦仲麒[1] 杨立[1] 程寅胜 LIU Zheng;AN Lin;WU Tao;QIN Zhong-qi;YANG Li;CHENG Yin-sheng(Institute of Fruit and Tea,Hubei Academy of Agricultural Science/Fruit and Tea Research Branch,Hubei Innovation Center of Agricultural Science and Technology,Wuhan 430064,China;Key Laboratory of Horticultural Plant Biology(Ministry of Education),Huazhong Agricultural University,Wuhan 430072,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所/湖北省农业科技创新中心果树茶叶研究分中心,武汉430064 [2]华中农业大学园艺植物生物学教育部重点实验室,武汉430072

出　　处：《湖北农业科学》2021年第23期168-171,共4页Hubei Agricultural Sciences

基　　金：国家重点研发计划资助项目(2020YFD1000202,2018YFD1000200);国家现代农业产业技术体系建设专项(CARS-29-17);湖北省农业科技创新中心重大研发项目(2020-620-000-002-05);国家自然科学基金项目(31801819);湖北省农业科技创新中心资助项目(2019-620-000-001-22)。

摘　　要：提高光合作用水平是提高果树产量潜力的有效途径。生物钟基因能够响应外界环境信号,并在光合作用中发挥调控作用。研究分析了不同类型梨品种(包括砂梨、白梨以及西洋梨)光合作用差异,检测了PpPRR5基因在不同品种中的表达水平变化模式。结果表明,不同梨品种的净光合速率存在显著差异,砂梨品种翠冠净光合速率最高,白梨品种居中,西洋梨品种阿巴特净光合速率最低。表达分析发现,与梨树光合作用密切相关的生物钟基因PpPRR5表达水平与不同品种光合作用能力呈负相关性,表明PpPRR5可能是梨树光合作用的负调控因子。Improvement in photosynthesis is an effective way to further increase yield potential in fruit crops.Circadian clock genes could be adjusted by environmental cues,and thereby regulate photosynthesis.The differences of photosynthesis of different types of pear varieties(including Pyrus pyrifolia,Pyrus bretschneider and western pear)were studied and analyzed,and the expression level and change pattern of PpPRR5 gene in different varieties were detected.The results showed that significantly different levels of net photosynthetic rate were observed in five pear genotypes.Pyrus pyrifolia Nakaicv.‘Cuiguan’had the highest levels of net photosynthetic rate.Pyrus bretschneider maintained moderate levels of net photosynthetic rate.Pyrus communis L.cv.‘Abate Fetel’showed the lowest levels of net photosynthetic rate.There was a negative correlation between the expression of circadian clock gene PpPRR5 and the capacity of pear genotype for photosynthetic rate.The result suggests that PpPRR5 acts as a negative regulator of photosynthetic performance.

关 键 词：梨树 光合作用 不同品种 PpPRR5 表达水平 

分 类 号：Q819[生物学—生物工程] S661.2[农业科学—果树学]