仿红茶调制工艺烟叶的评吸质量及差异化合物研究  

Smoking quality and differential compounds analysis for tobacco leaves prepared imitating black tea modulation process

作　　者：刘钰坤 周东波 汪耀富 沈程文 李彦 戴林建[1] LIU Yukun;ZHOU Dongbo;WANG Yaofu;SHEN Chengwen;LI Yan;DAI Linjian(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Technology Center,Hunan Zhongyan Co.,Ltd,Changsha,Hunan 410007,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]湖南中烟工业有限责任公司技术中心,湖南长沙410007

出　　处：《湖南农业大学学报（自然科学版）》2021年第6期642-647,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南中烟工业有限责任公司项目(202043000834081)。

摘　　要：以云烟87中部叶为原料,进行萎凋(烤房萎凋和覆膜萎凋)、揉捻(20 min)、发酵(6 h)、干燥(85~105℃两段式)仿红茶调制工艺试制烤烟。与对照三段式烤烟相比,仿红茶调制工艺烟叶香气细腻柔和,浓度中等,有甜香、干草香、焦香、烘烤香等特点;运用GC-MS和LC-MS检测烟叶化学成分,仿红茶调制工艺烟叶与对照样本中共鉴定到2380种差异化合物,占化合物总量的43.26%;主成分分析(PCA)与层聚类分析(HCA)表明,仿红茶调制工艺烟叶样本与对照样本中化合物含量存在显著差异,萎凋方式对仿红茶调制烟叶的评吸结果影响最大;覆膜萎凋评吸结果整体优于烤房萎凋的,干燥方式对特征香气的形成具有重要作用;相关性分析表明,2595种化合物含量与萎凋方式相关;通过正交偏最小二乘判别分析(OPLS-DA)差异检验方法鉴定到2708种标志性化合物,通过整合,2种萎凋方式共鉴定到关键差异化合物757种。The middle leaves of Yunyan87 were used as materials and processed imitating black tea method including withering(curing barn withering and film covered withering),rolling(20 min),fermentation(6 h)and drying(85-105℃two-stage method).Compared to conventional flue-cured tobacco with three-stage method,tobacco leaves prepared by imitating black tea method showed soft and subtle aroma,which was in moderate concentration,including sweet aroma,dry grass aroma,burnt aroma and baking aroma.GC-MS and LC-MS were used to test the chemical components of tobacco leaves,and the results showed that 2380 differential compounds were identified,accounting for 43.26%of the total compounds.Through principal component analysis(PCA)and hierarchical cluster analysis(HCA),it was found that there were significant differences in the content of compounds in tobacco leaves with two different curing methods.Withering method showed the biggest effect on smoking evaluation for tobacco samples prepared by imitating black tea method and plastic film covered withering showed better smoking quality.Drying method was important for the formation of characterized aroma.The correlation analysis showed that the contents of 2595 compounds were correlated with withering method.By orthogonal partial least squares discriminant analysis(OPLS-DA),2708 marker compounds were identified and 757 marker compounds were identified by T-test and ploidy change.Through integration of the data with two different withering methods,757 key differential compounds were identified.

关 键 词：烟草 仿红茶调制 评吸质量 差异化合物 

分 类 号：S572.092[农业科学—烟草工业]