幼苗期生菜耐镉性综合评价与低镉积累种质的筛选  

Comprehensive evaluation of cadmium tolerance in lettuce seedling and selection of low cadmium accumulation cultivars

作　　者：刘春 杜雪玲[1] 施昌倩 刘兰 夏雨晴 闫小玲 胡超猛 许慧娟 余如刚[1] LIU Chun;DU Xueling;SHI Changqian;LIU Lan;XIA Yuqing;YAN Xiaoling;HU Chaomeng;XU Huijuan;YU Rugang(College of Life Sciences,Huaibei Normal University,Huaibei,Anhui 235000,China)

机构地区：[1]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《湖南农业大学学报（自然科学版）》2021年第6期648-654,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：安徽省教育厅项目(KJ2020A0039);淮北师范大学引进博士科研启动经费(03106059)。

摘　　要：采用盆栽方式,对10个生菜品种(‘意大利’‘海南清脆玻璃’‘四季耐抽薹’‘耐热’‘大速’‘中国台湾绣球’‘日本结球’‘日本紫叶’‘红霞紫叶’‘法国紫红花叶’)进行Cd胁迫处理(CdCl_(2)·2.5H_(2)O含量分别为2、10 mg/kg),测定各处理植株叶片和根部的镉含量。以生菜幼苗株高、叶宽、根长、地上部干质量、根干质量、叶绿素含量的镉耐性系数为衡量耐Cd性依据,综合评价生菜的耐Cd能力:由主成分分析将生菜生长性状的耐镉系数变换为3个相互独立的综合指标,包含所有数据86.22%的内容;通过隶属函数法,计算生菜苗期Cd的耐受综合评价值(D),对生菜品种进行聚类,将10个生菜品种划分为耐Cd(‘法国紫花红叶’‘红霞紫叶’‘四季耐抽薹’)、中度耐Cd(‘日本紫叶’)、高度耐Cd(‘意大利’‘耐热’)以及Cd敏感(‘日本结球’‘海南清脆玻璃’‘大速’‘中国台湾绣球’)4种类型;通过逐步回归法构建生菜耐Cd性评价数学模型。试验结果表明,叶宽和株高对生菜耐Cd性影响显著,可以作为生菜耐Cd性评价的鉴定指标;‘意大利’‘耐热’的植株镉含量富集量较低,属于耐Cd、可食部位低Cd积累品种。Pot culture was used to treat 10 lettuce varieties(‘Yidali’,‘Hainanqingcuiboli’,‘Sijinaichoutai’,‘Naire’,‘Dasu’,‘Zhongguotaiwanxiuqiu’,‘Ribenjieqiu’,‘Ribenziye’,‘Hongxiaziye’,‘Faguozihonghuaye’)under Cd stress(CdCl_(2)2,10 mg/kg),and the cadmium content of leaves and roots of each plant was measured.The Cd tolerance of lettuce was comprehensively evaluated based on the stress tolerance coefficients of plant height,leaf width,root length,shoot dry mass,root dry mass and chlorophyll content of lettuce seedlings.The cadmium tolerance coefficient of lettuce growth traits was transformed into three independent comprehensive indexes by principal component analysis,including 86.22%of all data.Through the membership function method,the comprehensive evaluation value D of Cd tolerance of lettuce at seedling stage was calculated,and the lettuce varieties were clustered.The 10 lettuce varieties were divided into four types:Cd tolerance(‘Faguozihonghuaye’,‘Hongxiaziye’,‘Sijinaichoutai’),moderate Cd tolerance(‘Ribenziye’),high Cd tolerance(‘Yidali’,‘Naire’)and Cd sensitivity(‘Ribenjieqiu’,‘Hainanqingcuiboli’,‘Dasu’,‘Zhongguotaiwanxiuqiu’).The mathematical model of Cd tolerance evaluation of lettuce was constructed by stepwise regression method,which was results showed that leaf width and plant height had significant effects on Cd tolerance of lettuce,which can be used as identification indexes for Cd tolerance evaluation of lettuce.Yidali and Naire plants have low cadmium enrichment,belonging to Cd tolerant varieties with low Cd accumulation in edible parts.

关 键 词：生菜 幼苗期 耐镉性 主成分分析 隶属函数法 逐步回归法 

分 类 号：S636.201[农业科学—蔬菜学]