四妙丸提取物乙醇提取部位对肝脏脂质蓄积的作用  

Effects of ethanol extract of Si Miao Wan on hepatic lipid accumulation

作　　者：马晶杰 陈伊梦 姜琪欣 杨杰[1] 闻晓东[1] MA Jingjie;CHEN Yimeng;JIANG Qixin;YANG Jie;WEN Xiaodong(State Key Laboratory of Natural Medicines,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]中国药科大学中药学院,天然药物活性组分与药效国家重点实验室,南京211198

出　　处：《中国药科大学学报》2021年第6期725-734,共10页Journal of China Pharmaceutical University

基　　金：国家重点研发计划资助项目(No.2018YFC1704500,No.2018YFC1704503)。

摘　　要：研究四妙丸提取物的60%乙醇洗脱部位(ESMW)对肝脏脂质蓄积的改善作用及其机制。采用甘油三酯(TG)试剂盒、BODIPY荧光染色、QPCR、Westernblot、油红O染色、AMPKα敲减等方法检测四妙丸提取物ESMW对游离脂肪酸诱导的肝细胞脂质蓄积的改善作用;并在高脂饲喂小鼠中,通过检测ESMW对高脂饮食小鼠的口服糖耐量、血脂、肝脏脂质水平、肝组织脂代谢相关mRNA及蛋白表达等生化指标,验证ESMW对肝脏脂质蓄积的改善作用及其机制。结果表明,ESMW通过调节AMPK信号通路抑制游离脂肪酸诱导的肝原代细胞脂质堆积;显著改善了高脂饮食小鼠肝脏的脂质堆积,且该药效的发挥与AMPK信号通路有关。This study aimed to investigate the ameliorative effects of 60%ethanol elution fraction(ESMW)from Si Miao Wan on the hepatic lipid accumulation and its mechanism.TG kit,BODIPY fluorescence staining,QPCR,WB,oil red O staining,and AMPKαknockdown were used to detect the ability of ESMW to improve lipid accumulation in hepatocytes stimulated with free fatty acid.Furthermore,the effects of ESMW on the oral glucose tolerance,serum biochemical indexes,TG content in liver tissue,the expressions of mRNA and protein related to lipid metabolism in liver tissue were studied in mice fed with high fat diet to verify the mechanism of ESMW fraction on hepatic lipid accumulation.The results showed that ESMW inhibited lipid accumulation induced by free fatty acids by regulating AMPK signaling pathway,and that ESMW significantly improved the lipid metabolism of mice fed with high fat diet,with relation to AMPK signaling pathway.

关 键 词：四妙丸 乙醇提取物 糖脂代谢 AMPK 脂质蓄积 脂质合成 

分 类 号：R965[医药卫生—药理学]