荧光染色法在真菌检测中的应用  

Application of fluorescent staining in fungus detection

作　　者：赵琳[1] 乔昀[1] ZHAO Lin;QIAO Yun(Department of Clinical Laboratory,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)

机构地区：[1]上海中医药大学附属曙光医院检验科,上海200021

出　　处：《检验医学》2021年第12期1219-1221,共3页Laboratory Medicine

摘　　要：目的探讨荧光染色法在真菌检测中的应用价值。方法选取232份临床样本,采用荧光染色、直接涂片、革兰染色、墨汁染色、真菌培养及KOH湿片法同步检测。结果在175份痰、咽拭子、白带、尿液和粪便等深部样本中,荧光染色法真菌阳性检出率(87.43%)高于直接涂片法(78.86%)和革兰染色法(74.29%)(P<0.05)。在27份皮屑和甲屑浅表样本中,荧光染色法真菌阳性检出率(85.19%)高于KOH湿片法(59.26%)和真菌培养法(55.56%),差异有统计学意义(P<0.05)。在30份脑脊液样本中,荧光染色法与墨汁染色法真菌阳性检出率差异无统计学意义(P>0.05)。结论荧光染色法简便、快速、特异性高,在真菌检测中具有一定的价值。Objective To evaluate the application of fluorescent staining in fungus detection. Methods A total of 212 clinical samples were collected. Fluorescent staining,direct smear,Gram staining,ink staining,fungal culturing and KOH wet film were used. Results In 175 deep samples,including sputum,pharyngeal swab,leucorrhea,urine and feces,the positive rates of fungi by fluorescent staining,direct smear and Gram staining were 87.43%,78.86% and 74.29%,respectively. The positive rate of fluorescent staining was higher(P<0.05). In 27 superficial samples of dandruff and nail dandruff,the positive rates of fungi were 85.19%,59.26% and 55.56%by fluorescent staining,KOH wet film and fungal culturing,respectively(P<0.05). The positive rates of fungi by fluorescent staining and ink staining in 30 cerebrospinal fluid samples had no statistical significance(P>0.05).Conclusions Fluorescent staining is simple,rapid and specific,and it has certain clinical value in the diagnosis and identification of fungi.

关 键 词：真菌 荧光染色 直接涂片 革兰染色 真菌培养 KOH湿片法 墨汁染色 

分 类 号：R379[医药卫生—病原生物学]