重度子痫前期患者血清和胎盘组织中ADMA与DDAH2的表达水平及其相关性  

Expression Value of ADMA and DDAH2 in Early Onset Severe Preeclampsia

作　　者：王宇[1] 周杜娟 黄艳[1] 徐雁 夏宏林[1] WANG Yu;ZHOU Dujuan;HUANG Yan(Suzhou Hospital Affiliated to Anhui Medical University,Anhui Suzhou 234000,China)

机构地区：[1]安徽医科大学附属宿州医院,安徽宿州234000

出　　处：《河北医学》2021年第12期1965-1969,共5页Hebei Medicine

基　　金：国家自然科学基金项目,(编号:KJ2017A832)。

摘　　要：目的:观察重度子痫前期患者血清和胎盘组织中非对称性二甲基精氨酸(ADMA)与二甲基精氨酸二甲胺水解酶(DDAH)的表达水平,以及ADMA与DDAH的相关性。方法:前瞻性选取2017年1月至2020年10月安徽医科大学附属宿州医院收治的62例重度子痫前期患者作为研究对象,其中早发型重度子痫前期32例,晚发型重度子痫前期30例,将同期该院30例健康孕妇作为对照组,检测三组研究对象的血清ADMA与DDAH2表达水平及胎盘组织中ADMA与DDAH2表达阳性率,分析ADMA与DDAH2在血清中表达的相关性。结果:早发型重度子痫前期组外周血清ADMA水平显著高于晚发型重度子痫前期组及对照组,血清DDAH2水平则较晚发型重度子痫前期组及对照组低,差异有统计学意义(P<0.05);早发型重度子痫前期组胎盘组织ADMA阳性表达率(93.75%)明显高于晚发型重度子痫前期组(66.67%)及对照组(46.67%);胎盘组织DDAH2阳性表达率(37.50%)明显低于晚发型重度子痫前期组(66.67%)及对照组(80.00%),差异均有统计学意义(P<0.05)。Pearson相关系数分析显示,血清ADMA水平与DDAH2在早发型及晚发型重度子痫前期均呈现为负相关(r=-0.71,-0.77,P<0.05)。结论:与正常孕妇及晚发型子痫前期孕妇相比,早发型重度子痫前期患者ADMA呈现高表达,DDAH2呈现低表达,两者均参与了早发型重度子痫前期形成与发展,应予以重视。Objective:To observe the expression levels of asymmetric dimethylarginine(ADMA)and dimethylarginine dimethylamine hydrolase(DDAH)in serum and placenta of patients with severe preeclampsia,and the correlation between ADMA and DDAH.Methods:62 patients with severe preeclampsia admitted to Suzhou Hospital Affiliated to Anhui Medical University from January 2017 to October 2020 were prospectively selected as the research subjects,including 32 cases of early-onset severe preeclampsia and 30 cases of late-onset severe preeclampsia.30 healthy pregnant women in our hospital during the same period were selected as the control group.The serum ADMA and ddah2 expression levels and placental ADMA and ddah2 expression levels of the three groups were detected.The correlation between the expression of ADMA and ddah2 in serum was analyzed.Results:The level of serum ADMA in early-onset severe preeclampsia group was significantly higher than that in late-onset severe preeclampsia group and control group,and the level of serum ddah2 was lower than that in late-onset severe preeclampsia group and control group,with statistical significance(P<0.05);the positive expression rate of ADMA in placental tissue in early-onset severe preeclampsia group(93.75%)was significantly higher than that in late-onset severe preeclampsia group(66.67%)and control group(P<0.05).The positive expression rate of ddah2 in placenta(37.50%)was significantly lower than that in late-onset severe preeclampsia group(66.67%)and control group(80.00%)(P<0.05).Pearson correlation coefficient analysis showed that serum ADMA level was negatively correlated with ddah2 in early-onset and late-onset severe preeclampsia(r=-0.71,-0.77,P<0.05).Conclusion:Compared with normal pregnant women and late-onset preeclampsia pregnant women,early-onset severe preeclampsia patients showed high expression of ADMA and low expression of ddah2.Both of them were involved in the formation and development of early-onset severe preeclampsia,which should be paid attention to.

关 键 词：重度子痫前期 早发型 晚发型 非对称性二甲基精氨酸 二甲基精氨酸二甲 

分 类 号：R714.244[医药卫生—妇产科学]