针刺对偏头痛大鼠脑干IL-6、TNF-α及三叉神经脊束核CGRP水平的影响  

Effects of Acupuncture on Levels of Brainstem IL-6,TNF-αand Spinal Trigeminal Nucleus CGRP in Migraine Rats

作　　者：张亚兰 宋伯骐 贺煜竣 杨凌毓 付磊[1] 刘未艾[1] ZHANG Yalan;SONG Boqi;HE Yujun;YANG Lingyu;FU Lei;LIU Weiai(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《中国中医药信息杂志》2022年第1期59-64,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖南省自然科学基金面上项目(2019JJ40227);中国博士后科学基金面上项目(2017M612566);湖南中医药大学研究生科研创新项目(2018CX47);第四批全国中医(临床、基础)优秀人才研修项目(J20184832009);湖南省高层次卫生人才“225”工程培养资助项目(2018年)。

摘　　要：目的观察针刺"风池""外关""阳陵泉"对偏头痛模型大鼠脑干白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及三叉神经脊束核降钙素基因相关肽(CGRP)水平的影响,探讨其可能的作用机制。方法将SD大鼠随机分为空白组、模型组、针刺组和西药组,每组10只。第8日采用皮下注射硝酸甘油制备偏头痛大鼠模型。针刺组和西药组于造模前7d及造模后30min分别针刺双侧"风池""外关""阳陵泉"及灌胃盐酸氟桂利嗪溶液,每日1次,连续8 d。观察大鼠造模前后行为学变化及50%缩足阈值,干预结束后,免疫组化检测大鼠脑干IL-6、TNF-α表达,RT-qPCR检测大鼠三叉神经脊束核CGRPm RNA表达。结果与空白组比较,模型组、针刺组、西药组大鼠行为学评分明显增加(P<0.01),50%缩足阈值明显降低(P<0.01)。治疗后,与空白组比较,模型组大鼠脑干IL-6、TNF-α阳性细胞及三叉神经脊束核CGRP mRNA表达明显升高(P<0.01);与模型组比较,针刺组、西药组大鼠脑干IL-6、TNF-α阳性细胞及三叉神经脊束核CGRP mRNA表达明显降低(P<0.01);针刺组与西药组比较差异无统计学意义(P>0.05)。结论针刺"风池""外关""阳陵泉"可降低偏头痛大鼠脑干IL-6、TNF-α及三叉神经脊束核CGRP表达,这可能是其治疗偏头痛的作用机制之一。Objective To observe the effects of acupuncture at“Fengchi”,“Waiguan”and“Yanglingquan”on levels of brainstem IL-6,TNF-αand spinal trigeminal nucleus calcitonin gene related peptide(CGRP)in migraine model rats;To explore its possible mechanism.Methods SD rats were randomly divided into blank group,model group,acupuncture group and Western medicine group,with 10 rats in each group.The model of migraine rats was made by subcutaneous injection of nitroglycerin on the eighth day.Seven days before modeling and 30 minutes after modeling,acupuncture group and Western medicine group were treated with acupuncture at bilateral“Fengchi”,“Waiguan”“Yanglingquan”and flunarizine hydrochloride solution by gavage respectively,once a day,for eight consecutive days.The behavioral changes of rats were observed and 50%paw withdrawal threshold were measured before and after modeling.After treatment,the expression of IL-6 and TNF-αin the brainstem were detected by immunohistochemistry,the m RNA expression of CGRP in spinal trigeminal nucleus was detected by RT-q PCR.Results Compared with the blank group,the behavioral score of rats in the model group,acupuncture group and Western medicine group increased significantly(P<0.01),and the 50%paw withdrawal threshold decredsed significantly(P<0.01).Compared with the blank group,the brainstem IL-6,TNF-αpositive cells and spinal trigeminal nucleus CGRP mRNA expression of rats in the model group increased(P<0.01).Compared with the model group,the brainstem IL-6,TNF-αpositive cells and spinal trigeminal nucleus CGRP m RNA expression of rats in the acupuncture group and Western medicine group decreased(P<0.01).There was no significant difference between acupuncture group and Western medicine group(P>0.05).Conclusion Acupuncture at“Fengchi”“Waiguan”and“Yanglingquan”can reduce the expressions of IL-6,TNF-αin the brainstem and CGRP in the spinal trigeminal nucleus of migranie rats,which may be one of the mechanisms of treating migraine.

关 键 词：偏头痛 针刺 风池 外关 阳陵泉 降钙素基因相关肽 白细胞介素-6 肿瘤坏死因子-α 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]