茶花鸡PRLR基因多态性与产蛋性状的相关性研究  

Study on the Correlation between PRLR Gene Polymorphism and Egg Production Performance of Chahua Chickens

作　　者：孙大卫 柳明正 杜彦丽 张茹 自先念 何洋 王坤[1] 豆腾飞 贾俊静[1] 葛长荣[1] SUN Dawei;LIU Mingzheng;DU Yanli;ZHANG Ru;ZI Xiannian;HE Yang;WANG Kun;DOU Tengfei;JIA Junjing;GE Changrong(College of Animal Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《中国家禽》2021年第11期14-19,共6页China Poultry

基　　金：云岭学者及云岭产业技术领军人才培养经费;苏正昌专家工作站(2019IC008);云南省教育厅科学研究基金(2019Y0082);云南省科技计划项目重大科技专项(2016ZA008)。

摘　　要：为探究茶花鸡PRLR基因多态性与茶花鸡产蛋性状的相关性,挖掘影响茶花鸡产蛋性状的分子遗传标记,研究以300只300日龄的茶花鸡为研究对象,通过测序检测PRLR基因是否存在SNPs位点,并与茶花鸡产蛋性状进行关联分析,再通过实时荧光定量PCR验证PRLR基因在不同组织中的表达情况。研究结果显示:在茶花鸡PRLR基因中发现两个SNPs位点(C-40T和A+47G),在C-40T的位点中,优势等位基因为T,属于低度多态;关联分析显示该位点与300日龄产蛋数显著相关(P<0.05)。在A+47G位点中,优势等位基因为G,属于中度多态;关联分析显示该位点与开产日龄显著相关(P<0.05)。两个位点均显著偏离哈迪-温伯格平衡(P<0.05)。PRLR在茶花鸡高产组卵巢中的表达显著高于低产组(P<0.05),在高产组卵巢中的表达显著高于垂体(P<0.05)。综上所述,茶花鸡PRLR基因位点C-40T和A+47G可能分别是茶花鸡43周龄产蛋数和开产日龄的分子标记,为茶花鸡产蛋性能的选育提供参考依据。To explore the correlation between PRLR gene polymorphism and egg production traits of Chahua chicken and the molecular genetic markers affecting egg production of Chahua chicken,this experiment took 43-week-old Chahua chicken as materials,a total of 300 individuals,confirmed whether there were SNPs in PRLR gene by sequencing,and analyzed the association between PRLR gene and egg production traits of Chahua chicken,and verified the expression of PRLR gene in different tissues by real-time quantitative PCR.The results showed that two SNPs(C-40T and A+47G)were found in the PRLR gene of Chahua chicken.In C-40T locus,the dominant allele was T which belonged to low degree polymorphism.Association analysis showed that this locus was significantly correlated with the number of eggs laid at 300 days of age(P<0.05).In A+47G locus,the dominant allele was G,which belonged to moderate polymorphism.Association analysis showed that this locus was significantly correlated with the age of the first day of labor(P<0.05).Both loci deviated significantly from hardy Weinberg equilibrium(P<0.05).The expression of PRLR in ovary of high yield group was significantly higher than that of low yield group(P<0.05),and the expression of PRLR in ovary of high yield group was significantly higher than that of pituitary(P<0.05).In conclusion,the C-40T and A+47G loci of PRLR gene may be molecular markers of egg number at 43 weeks of age and age at first egg,respectively,which may provide a reference for the breeding of laying performance of Chahua chicken.

关 键 词：PRLR基因 茶花鸡 产蛋性状 关联分析 

分 类 号：S831.2[农业科学—畜牧学]