UPLC-MS/MS法检测驴胶补血颗粒中牛皮源及猪皮源成分  

Analysis of components from ox and pig skins in LYUjiao Buxue granules by UPLC-MS/MS

作　　者：郑文燕 叶小英 萧绮珊 钟志昭 贺凡珍 许润娟 ZHENG Wenyan;YE Xiaoying;XIAO Qishan;ZHONG Zhizhao;HE Fanzhen;XU Runjuan(Zhongshan Institute of Food and Drug Control,Zhongshan 528400,China;Zhongshan Modem Chinese Medicine Park Development Co.Ltd.Zhongshan 528400,China)

机构地区：[1]中山市食品药品检验所,广东中山528400 [2]中山市华南现代中医药城发展有限公司,广东中山528400

出　　处：《沈阳药科大学学报》2021年第11期1152-1158,共7页Journal of Shenyang Pharmaceutical University

基　　金：广东省中山市科技局2020年社会公益研究项目(2020B2065)。

摘　　要：目的建立超高效液相色谱-三重四级杆质谱法(UPLC-MS/MS法)测定驴胶补血颗粒中牛皮源、猪皮源成分。方法用胰蛋白酶对驴胶补血颗粒中胶类成分进行酶解,采用UPLC-MS/MS法,以牛皮源特征肽m/z 641.3(双电荷)→726.2、m/z 641.3(双电荷)→783.3及猪皮源特征肽m/z 774.5(双电荷)→977.8、m/z 774.5(双电荷)→1034.6作为检测离子对,离子化模式为ESI+,进行多反应监测。结果10批驴胶补血颗粒中,2批次仅检出牛皮源成分,2批次仅检出猪皮源成分,2批次同时检出牛皮源和猪皮源成分。结论所建立的检测方法专属性强,可用于驴胶补血颗粒中掺假杂皮胶的检测。Objective To establish a ultra-high performance liquid chromatography-triple quardrupole mass spectrometry(UPLC-MS/MS)method for identification of ox and pig skins in LYUjiaoBuxue granules.Methods Trypsin was used to enzymatically hydrolyze gelatins in LYUjiao Buxue granules,and characteristic peptide segments of ox and pig skins were dectected by UPLC-MS/MS.The positive electrospray ionization and multi reaction monitoring were adoped with detection ion pairs as follows:m/z 641.3(double charge)→726.2 and m/z 641.3(double charge)→783.3 for ox skin,m/z 774.5(double charge)→977.8 and m/z 774.5(double charge)→1034.6 for pig skin.Results 10 batches of samples were assayed.2 batches were detected the characteristic peptide segments of ox skin,2 batches were detected the characteristic peptide segments of pig skin,another 2 batches were detected both ox and pig skins at the same time.Conclusion The method is specific and can be used for the determination of adulteration in LYUjiao Buxue granules.

关 键 词：驴胶补血颗粒 UPLC-MS/MS 牛皮源 猪皮源 

分 类 号：R917[医药卫生—药物分析学]