南四湖人工湿地沉积物中氨氧化细菌的筛选和鉴定  

Separation and identification of ammonia-oxidizing bacteria in sediments of Nansi Lake artificial wetland

作　　者：张念鑫 孔勇 于君君 舒凤月[1] 周晶[1] 王庆峰 于国红 ZHANG Nianxin;KONG Yong;YU Junjun;SHU Fengyue;ZHOU Jing;WANG Qingfeng;YU Guohong(College of Life Sciences,Qufu Normal University,Qufu 273165,China;Shanghai Academy of Agricultural Sciences,Shanghai 201306,China;Dry-Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences,Hengshui 053000,China)

机构地区：[1]曲阜师范大学生命科学学院,曲阜273165 [2]上海市农业科学院,上海201403 [3]河北省农林科学院旱作农业研究所,衡水053000

出　　处：《上海农业学报》2021年第6期103-107,共5页Acta Agriculturae Shanghai

基　　金：国家自然科学青年基金项目(41807053)。

摘　　要：氮污染已成为影响南四湖水质的重要因素之一,为了解南四湖沉积物中可培养氨氧化细菌的种类和系统发育信息,采用富集培养的方法,从南四湖人工湿地沉积物中筛选出具有氨氮去除能力的细菌,并用格利斯试剂检测细菌氨氮去除能力的强弱,从沉积物中筛选出高效的氨氧化细菌,并对其进行16S rDNA序列系统进化树的构建及分析。结果筛选到5株氨氧化细菌,经鉴定:3号和11号属于异氧硝化菌(Diaphorobacter sp.)、5号属于嗜酸菌(Acidovorax ebreus)、4号和12号属于酯香微杆菌(Microbacterium esteraromaticum)。从南四湖人工湿地沉积物中分离出的5株氨氧化菌,既可利用有机氮又可利用无机氮,其营养来源多样,适应力极强。该结论不仅为研究南四湖内部的氮素循环起重要作用,还为污染水体的生物修复提供了菌株材料。Nitrogen pollution has become an important factor affecting water quality of Nansi Lake.In this study,bacteria with ammonia nitrogen removal ability were screened from the sediments of the constructed wetland of Nansi Lake to provide materials for promoting the nitrogen cycle of the polluted water.The bacteria were cultured by enrichment culture method,and the removal ability of ammonia nitrogen of bacteria was detected by Griess reagent.Efficient ammonia-oxidizing bacteria were screened from the sediments,and the phylogenetic tree of 16S rDNA sequence was constructed and analyzed.In this experiment,a total of 5 strains of ammonia-oxidizing bacteria were screened,and the results after identification were 2 strains of Diaphorobacter sp,1 strain of Acidovorax ebreus,and 2 strains of Microbacterium esteraromaticum.Five strains of ammonia-oxidizing bacteria isolated from the sediments of Nansi Lake artificial wetland can make use of both organic and inorganic nitrogen.It plays an important role in the nitrogen cycle of Nansi Lake and provides bacterial material for bioremediation of polluted water.

关 键 词：南四湖人工湿地 氨氧化菌 氮素循环 富集培养 

分 类 号：S181[农业科学—农业基础科学] Q938.1[生物学—微生物学]