用人工哺乳新生仔猪进行临床分离PEDV-XS12的LD_(50)测定  

LD_(50) determination of clinically isolated PEDV-XS12 in artificially suckling newborn piglets

作　　者：王剑 徐慧明 门程芳 徐一凡 于瑞嵩[1] 董世娟[1] 谢春芳[1] 李震[1] WANG Jian;XU Huiming;MEN Chengfang;XU Yifan;YU Ruisong;DONG Shijuan;XIE Chunfang;LI Zhen(Institute of Animal Husbandry&Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China)

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海市农业遗传育种重点实验室,上海201106

出　　处：《上海农业学报》2021年第6期114-122,共9页Acta Agriculturae Shanghai

基　　金：上海市科技兴农攻关项目[沪农科攻字(2015)第6-1-9号]。

摘　　要：为测定临床流行的猪流行性腹泻病毒(Porcine Epidemic Diarrhea Virus,PEDV)-XS12毒株对新生仔猪的半数致死量(LD_(50)),了解不同攻毒剂量对新生仔猪攻毒后体征指标的影响,探索新生仔猪PEDV攻毒模型的建立,依次对PEDVAg-∕Ab-未哺乳新生仔猪进行PEDV肠毒增殖、肠毒扩增后的病毒载量测定和攻毒后新生仔猪的LD_(50)测定。测定过程中对采食量、体重、体温、24 h排泄量、粪便病毒载量等指标进行24 h、为期7 d的观察与记录,并通过固定剂量法(Fixed Dose Procedure,FDP)、序贯法(Up and Down Procedure,UDP)、改进寇氏法、Bliss法综合计算出PEDV-XS12 LD_(50)。最终,各组攻毒剂量分别为:200 copies∕mL(LD_(100)),126 copies∕mL(LD_(80)),79 copies∕mL(LD_(60)),50 copies∕mL(LD_(40)),31 copies∕mL(LD_(20)),20 copies∕mL(LD_(0))。各组受试动物死亡率依次为LD_(100)=10∕10、LD_(80)=6∕10、LD_(60)=5∕10、LD_(40)=4∕10、LD_(20)=3∕10、LD_(0)=2∕10。由此得出:PEDV-XS12 LD_(50)为48.044 copies∕mL,95%置信区间为29.053—72.071,i=0.63。该结果符合正态分布。The aim of this paper was to establish the Porcine Epidemic Diarrhea Virus(PEDV)-XS12 challenging model of newborn piglets through determining the median lethal dose(LD_(50))of the PEDV-XS12 strain to newborn suckling piglet.In this study,PEDVAg-∕Ab-newborn sucking piglets were fed back with PEDV-XS12 which was in order to proliferate more PEDV-XS12 copies.The quantity of PEDV-XS12 virons were determined before challenging the piglets for the LD_(50)determination.The physiological parameters of challenged piglets including food intake,body weight,body temperature,24 hours excretion and fecal viral copies were checked for 24 hours×7 days during the challenging experiment.Finally,PEDV-XS12 LD_(50)was calculated using the methods of fixed dose procedure(FDP),up and down procedure(UDP),modified Karber and Bliss.The following challenging doses were determined after several preliminary tests:200 copies∕mL(LD_(100)),126 copies∕mL(LD_(80)),79 copies∕mL(LD_(60)),50 copies∕mL(LD_(40)),31 copies∕mL(LD_(20)),20 copies∕mL(LD_(0)).After the challenging,mortality rate of the piglets with each challenging dose were LD_(100)=10∕10,LD_(80)=6∕10,LD_(60)=5∕10,LD_(40)=4∕10,LD_(20)=3∕10,LD_(0)=2∕10.Finally,LD_(50)of the PEDV-XS12 strain was 48.044 copies∕mL.The 95%confidence interval of PEDV-XS12 LD_(50)was 29.053-72.071,i=0.63.The result conformed to a normal distribution.

关 键 词：新生仔猪 PEDV流行毒株 LD_(50) 动物模型 

分 类 号：S858.[农业科学—临床兽医学]