枸杞多糖对Aβ_(1-42)诱导的小胶质细胞增殖、表型和分泌活性的影响  

Effect of Lycium barbarum polysaccharides on proliferation,phenotype and secretion activity of microglia induced by Aβ_(1-42)

作　　者：郭鹏[1] 单铁强[3] 任海霞[4] 宋亚娟[2] 马景红[4] 郭晓会[4] GUO Peng;SHAN Tie-qiang;REN Hai-xia;SONG Ya-juan;MA Jing-hong;GUO Xiao-hui(Department of Neurology,Handan Central Hospital,Handan Hebei,056002,China;Department of Clinical Laboratory,Handan Central Hospital,Handan Hebei,056002,China;Department of Clinical Laboratory,Qinhuangdao Haigang Hospital,Qinhuangdao Hebei,066003,China;Department of Gastroenterology,Affiliated Hospital of Hebei Engineering University,056002,China)

机构地区：[1]邯郸市中心医院神经内科,河北邯郸056002 [2]邯郸市中心医院检验科,河北邯郸056002 [3]秦皇岛市海港医院检验科,河北秦皇岛066003 [4]河北工程大学附属医院消化内科,河北邯郸056002

出　　处：《职业与健康》2021年第23期3186-3189,共4页Occupation and Health

摘　　要：目的探究枸杞多糖(lycium barbarum polysaccharide,LBP)对β淀粉样蛋白1-42(amyloid beta peptide 1-42,Aβ_(1-42))诱导的小胶质细胞增殖、表型和分泌活性的影响。方法体外培养小胶质细胞株BV2,被设计为4组:对照组、Aβ组、LBP组和LBP+Aβ组。对照组:加培养液;Aβ组:加培养液和5μmol/L的Aβ_(1-42);LBP组:加培养液和100μg/mL的LBP;LBP+Aβ组:提前用培养液和100μg/mL的LBP干预24 h,再加入5μmol/L的Aβ_(1-42);4组的干预时间均为8 h。噻唑蓝法估计4组细胞数目的变化情况,血清酶联免疫吸附试验技术测量培养悬浮液中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-1β(interleukin-1β,IL-1β)及白介素-10(interleukin-10,IL-10)的含量,免疫印迹技术分析细胞的表面分子白细胞分化抗原86(differentiation antigen 86,CD86)和精氨酸酶1(arginase-1,Arg-1)的含量。结果 LBP组和对照组相比,细胞数目,培养悬浮液中TNF-α、IL-1β和IL-10的含量,细胞CD86和Arg-1的表达水平差异均无统计学意义(均P>0.05);与对照组相比,Aβ组的细胞数目减少,TNF-α(184.98±16.30)、IL-1β(98.30±7.16)的量增加而IL-10(30.53±3.45)减少(t=18.219、12.891、10.721,均P<0.05),CD86(0.932±0.024)增加而Arg-1(0.213±0.013)减少(t=15.658、25.484,均P<0.05);和Aβ组组相比,LBP+Aβ组的细胞数目增多,TNF-α(68.09±7.53)、IL-1β(55.91±5.61)的量减少而IL-10(51.57±5.86)增加(t=15.946、11.415、7.579,均P<0.05),CD86(0.782±0.017)减少而Arg-1(0.402±0.020)增加(t=12.493、19.408,均P<0.05)。结论 Aβ_(1-42)能够诱导小胶质细胞数目减少,促进细胞分泌TNF-α、IL-1β和表达CD86,抑制细胞分泌IL-10和表达Arg-1,而枸杞多糖能够拮抗上述指标的改变。Objective To explore the effects of Lycium barbarum polysaccharide(LBP) on the proliferation,phenotype and secretion activity microglia induced by amyloid beta peptide 1-42(Aβ_(1-42)). Methods Microglia cell line BV2 was cultured in vitro and arranged into four groups:control group,Aβ group,LBP group and LBP+Aβ group. Control group:culture medium was added;Aβ group:culture medium and 5 μmol/L Aβ_(1-42) were added;LBP group:culture medium and 100 μg/mL LBP were added;LBP+Aβ group:culture medium and 100 μg/mL LBP were used in advance for 24 hours,then 5 μmol/L Aβ_(1-42) were added. The intervention time of the four groups was 8 hours. The changes of cell number in four groups were estimated by thiazole method. The contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β) and interleukin-10(IL-10) in culture suspension were measured by serum enzyme linked immunosorbent assay. The contents of leukocyte differentiation antigen 86(CD86) and arginase-1(Arg-1) were analyzed by western blot method. Results The differences in the cell number,the contents of TNF-α,IL-1β and IL-10 in culture suspension,and the expression levels of CD86 and Arg-1 were not statistically significant between LBP group and control group(all P>0.05). Compared with control group,the cell number in Aβ group decreased,the contents of TNF-α(184.98±16.30) and IL-1β(98.30±7.16) increased while the content of IL-10(30.53±3.45) decreased(t=18.219,12.891,10.721,all P<0.05),the level of CD86(0.932±0.024) increased while the level of Arg-1(0.213±0.013) decreased(t=15.658,25.484,both P<0.05). Compared with Aβ group,the cell number in LBP+Aβ group increased,the contents of TNF-α (68.09±7.53 ) and IL-1β (55.91±5.61)decreased while the content of IL-10(51.57±5.86) increased(t=15.946,11.415,7.579,all P<0.05),the level of CD86(0.782±0.017) decreased while the level of Arg-1(0.402±0.020) increased(t=12.493,19.408,both P<0.05). Conclusion Aβ_(1-42) can reduce the number of microglia,promote the secretion of TNF-α and IL-1β

关 键 词：枸杞多糖 小胶质细胞 增殖 表型 分泌活性 

分 类 号：R446.61[医药卫生—诊断学]