不同针灸方法对膝骨关节炎大鼠骨桥蛋白、基质金属蛋白酶-3和转化生长因子-β1表达的影响  

Effect of Different Acupuncture Methods on the Expression of OPN,MMP-3,TGF-β1 in Rats with Knee Osteoarthritis

作　　者：黄滢 何智军 庄礼兴[3] HUANG Ying;HE Zhi-jun;ZHUANGLi-xing(Guangzhou University of Traditional Chinese Medicine,Guangzhou Guangdong 510080;Guangzhou Panyu Hospital of Traditional Chinese Medicine,Guangzhou Guangdong 511400;The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou Guangdong 510080)

机构地区：[1]广州中医药大学,广东广州510080 [2]广州番禺中医院,广东广州511400 [3]广州中医药大学第一附属医院,广东广州510080

出　　处：《世界中西医结合杂志》2021年第10期1820-1825,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：2019年国家自然科学基金面上项目(81873327)。

摘　　要：目的探究不同针灸方法对膝骨关节炎(knee osteoarthritis,KOA)大鼠骨桥蛋白(osteopontin,OPN)、基质金属蛋白酶-3(MMP-3)和转化生长因子-β1(TGF-β1)表达的影响。方法将60只健康SD大鼠随机分为正常组、模型组、针刺组、火针组、温针组、电针组,每组10只。除正常组外,其余大鼠采用木瓜蛋白酶关节腔注射法构建KOA大鼠模型。正常组与模型组均不采取任何方法治疗。针刺组、火针组、温针组、电针组大鼠均选取内膝眼、外膝眼、阴陵泉穴,分别采用单纯针灸针进行针刺、火针、温针、电针进行治疗。评估各组膝关节严重程度指数(Lequesne MG),检测并比较治疗后KOA大鼠痛阈值、外周血和关节滑液OPN、MMP-3、TGF-β1表达水平。结果造模后,模型组、针刺组、火针组、温针组、电针组Lequesne MG评分均高于正常组,差异均有统计学意义(P<0.05)。治疗后,针刺组、火针组、温针组、电针组Lequesne MG评分均低于本组治疗前,差异有统计学意义(P<0.05),且针刺组、火针组、温针组、电针组Lequesne MG评分均低于模型组,差异有统计学意义(P<0.05),其中温针组、电针组Lequesne MG评分均低于针刺组,差异均有统计学意义(P<0.05)。治疗后,模型组痛阈值均低于正常组、针刺组、火针组、温针组、电针组,差异有统计学意义(P<0.05);且电针组痛阈值均高于正常组、模型组、针刺组、火针组、温针组,差异有统计学意义(P<0.05)。模型组和针刺组、火针组、温针组、电针组外周血OPN、MMP-3、TGF-β1 mRNA相对表达量均高于正常组,差异有统计学意义(P<0.05);针刺组、火针组、温针组、电针组外周血OPN、MMP-3、TGF-β1 mRNA相对表达量均低于模型组,差异有统计学意义(P<0.05)。模型组和针刺组、火针组、温针组、电针组外周血OPN、TGF-β1水平均高于正常组,差异有统计学意义(P<0.05);模型组、针刺组、火针组外周血MMP-3水平均高Objective To explore the effects of different acupuncture methods on osteopontin(OPN),matrix metalloproteinase-3(MMP-3)and transforming growth factor-β1(TGF-β1)in rats with knee osteoarthritis(KOA).Methods 60 clean 3-month-old healthy SD rats were equally divided into six group,normal group,model group,acupuncture group,fire acupuncture group,warm acupuncture group and electro-acupuncture group,with 10 rats in each group.Except for the normal group,other rats were injected with papain into the joint cavity to construct a KOA rat model.Neither the normal group nor the model group took any treatment.Rats in the acupuncture group,fire needling group,warm needling group,and electroacupuncture group were all selected for the inner knee eye,outer knee eye,and Yinlingquan points,and were treated with acupuncture,fire needling,warm needling,and electroacupuncture respectively with simple acupuncture needles.The knee joint severity index(Lequesne MG)of each group was evaluated,and the pain threshold,OPN,MMP-3,and TGF-β1 expression levels in peripheral blood and synovial fluid of KOA rats were detected and compared after treatment.Results After modeling,the Lequesne MG scores of the model group,acupuncture group,fire needle group,warm needle group,and electroacupuncture group were all higher than those of the normal group,and the differences were statistically significant(P<0.05).After treatment,the Lequesne MG scores of the acupuncture group,fire needling group,warm needling group,and electroacupuncture group were all lower than those of this group before treatment,the difference was statistically significant(P<0.05),and the acupuncture group,fire needling group,warm needling group The Lequesne MG scores in the electroacupuncture group were lower than the model group,and the difference was statistically significant(P<0.05).The Lequesne MG scores in the warm acupuncture group and the electroacupuncture group were lower than the acupuncture group,and the differences were statistically significant(P<0.05).After treatment,

关 键 词：膝骨关节炎 针灸 骨桥蛋白 基质金属蛋白酶-3 转化生长因子β1 

分 类 号：R285.5[医药卫生—中药学]