老年肺炎患者外周血单个核细胞端粒酶活性与气道脱落细胞高尔基体应激水平的相关性  

Correlation between telomerase activity of peripheral blood mononuclear cells and Golgi stress level of airway exfoliated cells in elderly patients with pneumonia

作　　者：贾李侠 白翠林 纪玉强[2] 张彦海 JIA Li-xia;BAI Cui-lin;JI Yu-qiang(Department of Cadre Ward,The 986th Air Force Hospital,Xi'an Shaanxi 710000,China;Department of Heart Research,Xi'an First Hospital,Xi'an Shaanxi 710002,China)

机构地区：[1]空军第九八六医院干部病房,陕西西安710000 [2]西安市第一医院心研室,陕西西安710002

出　　处：《临床和实验医学杂志》2021年第23期2519-2523,共5页Journal of Clinical and Experimental Medicine

基　　金：陕西省重点研发计划项目(一般项目)(编号:2021SF-151)。

摘　　要：目的探讨老年肺炎患者外周血单个核细胞端粒酶活性与气道脱落细胞高尔基体应激水平的相关性。方法回顾性纳入2018年6月至2021年6月在空军第九八六医院住院治疗的60例肺炎患者,根据年龄将患者分为老年组(≥65岁,n=37)和非老年组(<65岁,n=23),选取同期健康体检的20例志愿者作为对照组。通过酶联免疫吸附试验(ELISA)试剂盒测定所有标本中的端粒酶活性。通过四甲基偶氮唑盐(MTT)检测外周血单核细胞的增殖活性。通过免疫发光测定法和流式细胞仪技术分别检测髓过氧化物酶(MPO)和活性氧(ROS)活性。分析端粒酶活性与气道脱落细胞高尔基体MPO水平的相关性。并采用ELISA法测定血浆细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6和IL-5水平。结果端粒酶在PHA-M刺激前,老年组较非老年组和对照组端粒酶活性降低,差异有统计学意义(P<0.05),对照组和非老年组端粒酶活性比较差异无统计学意义(P>0.05)。端粒酶在PHA-M刺激后,老年组较非老年组和对照组端粒酶活性降低,非老年组较对照组端粒酶活性降低,差异均有统计学意义(P<0.05)。老年组和非老年组较对照组的端粒酶活性增加率降低,差异有统计学意义(P<0.05),老年组和非老年组端粒酶活性增加率比较差异无统计学意义(P>0.05)。活化24 h和48 h后,老年组和非老年组较对照组的增殖活性降低,老年组较非老年组增殖活性降低,差异均有统计学意义(P<0.05)。老年组和非老年组较对照组中MPO和ROS活性升高,非老年组较对照组MPO和ROS活性升高,差异均有统计学意义(P<0.05)。老年肺炎患者外周血单个核细胞端粒酶活性与气道脱落细胞高尔基体MPO水平负相关(r=-0.654,P<0.05)。老年组和非老年组较对照组血浆TNF-α、IL-6和IL-5水平升高,老年组较非老年组TNF-α、IL-6和IL-5水平升高,差异均有统计学意义(P<0.05)。结论老年肺炎患者外周血单个核Objective To investigate the correlation between the telomerase activity of peripheral blood mononuclear cells and the Golgi stress level of airway exfoliated cells in elderly patients with pneumonia.Methods This study retrospectively included 60 patients with pneumonia who were hospitalized in the 986 th Air Force Hospital from June 2018 to June 2021.The patients were divided into elderly group(≥65 years old,n=37)and non-elderly group(<65 years old,n=23)according to their ages,and 20 volunteers who received physical examinations during the same period were selected as control group.The enzyme-linked immunosorbent assay(ELISA)kit was adopted to determine the telomerase activity in all specimens.The proliferation activity of peripheral blood mononuclear cells was detected by MTT.The activity of MPO and ROS were detected by immunoluminescence assay and flow cytometry technology,respectively.The correlation between telomerase activity and Golgi MPO level in airway exfoliated cells was analyzed.The levels of plasma cytokines tumor necrosis factor alpha(TNF-α),interleukin(IL)-6 and IL-5 were determined by ELISA.Results Before PHA-M stimulation,the telomerase activity of the elderly group was lower than that of the non-elderly group and the control group,the differences were statistically significant(P<0.05),and there was no difference in telomerase activity between the control group and the non-elderly group(P>0.05).After telomerase was stimulated by PHA-M,the telomerase activity of the elderly group was lower than that of the non-elderly group and the control group,and the telomerase activity of the non-elderly group was lower than that of the control group,the differences were statistically significant(P<0.05).The increase rate of telomerase activity in the elderly group and the non-elderly group was lower than that of the control group,the difference was statistically significant(P<0.05),and there was no difference in the increase rate of telomerase activity between the elderly group and the non-elderly group(P>

关 键 词：老年肺炎患者 外周血单个核细胞 端粒酶 高尔基体 氧化应激 

分 类 号：R563.1[医药卫生—呼吸系统]