高剂量糖皮质激素对成年雄性小鼠PDGF-BB分泌及H型血管生长的作用研究  

Effects of high dose glucocorticoids on PDGF-BB secretion and H type vascular growth in adult male mice

作　　者：李素丽[1] 詹先琴 张艳君[1] 马艳荣[1] 王英[1] 王燕[1] LI Suli;ZHAN Xianqin;ZHANG Yanjun;MA Yanrong;WANG Ying;WANG Yan(Xinjiang Uygur Autonomous Region People's Hospital,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院,新疆乌鲁木齐830001

出　　处：《中国骨质疏松杂志》2021年第12期1752-1756,共5页Chinese Journal of Osteoporosis

基　　金：新疆维吾尔自治区自然科学基金面上项目(2017D01C104)。

摘　　要：目的探讨长期高剂量糖皮质激素对雄性成年小鼠血小板衍化生长因子-BB(PDGF-BB)分泌的影响以及对骨成长特异H型血管的作用。方法选取雄性3月龄C57小鼠,通过腹腔注射强的松龙10 mg/m^(2),持续四周,建立糖皮质激素性骨质疏松症组(GIOP组),同时设定对照组。测定血清骨形成标志物骨钙素(OC)、骨特异性碱性磷酸酶(BSAP)、胶原氨基酸延长肽I(PINP)及骨吸收标志物血清C端交联肽(CTX);测定骨髓上清和血清的PDGF-BB;对股骨行Micro-CT扫描;将股骨冰冻切片,行CD31^(hi)Emcn^(hi)H型血管免疫荧光染色。结果GIOP组血清中CTX-1表达上升,BSAP、OC和PINP表达下降,股骨Micro CT显示GIOP组相对于对照组骨量减少,GIOP组血清和骨髓中的PDGF-BB表达明显下降,CD31^(hi)和Endomucin^(hi)表达均有所下调。结论长期高剂量糖皮质激素对成年雄性小鼠破骨前体细胞PDGF-BB分泌有抑制作用,对骨成长特异的H型血管生长有抑制作用。骨特异的H型血管生长被抑制是糖皮质激素性骨质疏松症的发病机制之一。Objective To investigate the effects of long-term high-dose glucocorticoids on platelet derived growth factor-BB(PDGF-BB)secretion and bone growth specific H-type blood vessels in male adult mice.Methods 3-month-old male C57 mice were intraperitoneally injected with prednisone 10mg/m^(2) for 4 weeks(GIOP)as while control group was be set.Serum bone formation markers osteocalcin(OC),bone specific alkaline phosphatase(BSAP),and collagen amino acid prolongation peptide I(PINP)and bone resorption markers as serum C-terminal cross-linking peptide(CTX)were determined,PDGF-BB in bone marrow and serum were measured.Femur bone mineral density was scanned by micro-CT.The femur was frozen and CD31^(hi)Emcn^(hi)H type blood vessel immunofluorescence staining was performed.Results The serum expressions of CTX-1 was increased in the GIOP group,while the expressions of BSAP,OC and PINP were decreased compared by control group.Compared with the control group,the bone mass in the femoral Micro CT GIOP group was decreased,the expression of PDGF-BB in serum and bone marrow was significantly decreased in the GIOP group,and the expressions of CD31^(hi) and Endomucin^(hi) were downregulated.Conclusion Long-term high-dose glucocorticoid has inhibitory effect on PDGF-BB secretion of osteoclast precursor cells in adult male mice and has inhibitory effect on H-type blood vessel growth specific to bone growth.Inhibition of bone-specific H-type vascular growth is one of the pathogenesis mechanisms of glucocorticoid-induced secondary osteoporosis.

关 键 词：糖皮质激素性骨质疏松症 血小板衍化生长因子-BB H型血管 

分 类 号：R3[医药卫生—基础医学]