嗜碱性粒细胞活化试验在过敏性疾病应用中的研究进展  

Research progress of basophil activation test and its application in allergic diseases

作　　者：张敏 崔振泽[1] 李悦[1] 徐超[1] ZHANG Min;CUI Zhen-Ze;LI Yue;XU Chao(Dalian Children's Hospital,Dalian 116000,China)

机构地区：[1]大连市儿童医院,大连116000

出　　处：《中国免疫学杂志》2021年第22期2803-2809,共7页Chinese Journal of Immunology

基　　金：大连市医学科学研究计划项目(1911067)。

摘　　要：21世纪以来,过敏性疾病的发病率逐年增高,严重威胁着人类的生命健康。然而临床中针对这一类疾病目前没有安全高效的检测方法。本文通过梳理过敏性疾病及嗜碱性粒细胞活化试验的相关文献资料,认为该试验是一种安全高效的辅助诊断过敏性疾病的方法,其理论依据是嗜碱性粒细胞的表面标志物会随着过敏反应的发生而变化。基于这一理论依据,传统的嗜碱性粒细胞活化试验是利用流式细胞术对细胞进行检测,进而体外辅助诊断过敏性疾病。近年来基于微流体技术的新型嗜碱性粒细胞活化试验逐步兴起。流式细胞术是一种对细胞进行快速定量分析和筛选的方法。微流体技术是一种高精度处理小体积流体的技术,具有成本低、操作简便且所需样本及试剂量少的优点。本文就嗜碱性粒细胞的相关概念、分别基于流式细胞术及微流体技术的嗜碱性粒细胞活化试验及其在临床过敏性疾病中的应用价值做一综述,以期为嗜碱性粒细胞活化试验在过敏性疾病诊断领域的应用提供理论支持。Since the 21st Century,the incidence rate of allergic diseases has increased year by year,which seriously threatens human life and health.However,there is no safe and efficient detection method for this kind of disease.Many literatures about allergic diseases and basophil activation test have reported that basophil activation test is a safe and efficient method for the diagnosis of allergic diseases.And the theoretical basis of this test is that the surface markers of basophils will change with the occurrence of allergic reactions.Based on this theoretical basis,the traditional basophil activation test is to detect cells by flow cytometry,and then assist in the diagnosis of allergic diseases in vitro.In recent years,a new type of basophil activation test based on microfluidic technology has been developed.Flow cytometry is a rapid method for quantitative analysis and screening of cells.Microfluidic technology is a high precision technology for processing small volume fluid,which has the advantages of low cost,simple operation and small amount of samples and reagents.In this paper,the related concepts of basophils,basophil activation test which based on flow cytometry and microfluidic technology,and its application value in the diagnosis of allergic diseases are reviewed in order to provide theoretical support for the application of basophil activation test in the field of allergic diseases diagnosis.

关 键 词：嗜碱性粒细胞 流式细胞术 微流体技术 嗜碱性粒细胞活化试验 过敏性疾病 

分 类 号：R392.8[医药卫生—免疫学]