阿胶强骨口服液对大鼠缺铁性贫血的改善及其对铁代谢的调节作用机制初探  

Ejiao Qianggu oral liquid improves iron deficiency anemia in rats and mechanism of iron metabolism regulation

作　　者：恰木斯亚·吐尔逊哈里 美拉依·吉恩斯 武嘉林 Qiamusiya·Tuerxunhali;Meilayi·Jiensi;WU Jia-lin(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830011;Xinjiang Huashidan Pharmaceutical Co.,Ltd.,Urumqi 830011)

机构地区：[1]新疆医科大学中医学院,乌鲁木齐830011 [2]新疆华世丹药业股份有限公司,乌鲁木齐830011

出　　处：《中南药学》2021年第12期2549-2554,共6页Central South Pharmacy

基　　金：兵团专利运用与产业化项目(No.2018BD002)。

摘　　要：目的建立缺铁性贫血大鼠模型,观察阿胶强骨口服液对缺铁性贫血大鼠的作用效果,并初步探讨其作用机制。方法低铁饲料结合耳缘静脉放血建立缺铁性贫血大鼠模型,28 d后选取血红蛋白(HGB)<90 g·L^(-1)的大鼠分组给药。连续干预28 d,观察动物状态,并检测血常规HGB、红细胞数(RBC)、红细胞比容(HCT)、红细胞平均血红蛋白量(MCH)、红细胞平均血红蛋白浓度(MCHC)、红细胞平均体积(MCV)、血清铁(SI),ELISA法检测大鼠二价金属转运蛋白(DMT-1)和膜铁转运蛋白(FPN-1)含量。结果造模4周后模型对照组大鼠RBC、HGB、HCT、MCH、MCHC、MCV显著降低(P<0.05)。药物干预4周后,与模型对照组比较,阿胶强骨口服液各剂量组大鼠HGB、HCT、MCH、MCHC、MCV均显著增加(P<0.05),并趋于正常对照组水平;阿胶强骨口服液中、高剂量组大鼠SI(P<0.05)和DMT-1蛋白含量显著增加(P<0.01);高剂量组大鼠FPN-1蛋白含量显著增加(P<0.01)。结论阿胶强骨口服液对缺铁性贫血大鼠具有显著的治疗效果,既能提高血红蛋白含量,又能提高血清铁含量,其作用机制与肠道DMT-1与FPN-1密切相关。Objective To establish the rat model of iron deficiency anemia,determine the effect of Ejiao Qianggu oral liquid on the rats model of iron deficiency anemia,and discuss its mechanism.Methods Rat models of iron deficiency anemia were established by low iron diet combined with auricular vein bloodlet.Rats with hemoglobin (HGB)<90 g·L^(-1) were selected after 28 days.After continuous intervention for 28 days,the rat status was observed.Blood routine HGB,red blood cell (RBC),hematocrit (HCT),mean corpuscular hemoglobin (MCH),mean corpuscular hemoglobin concentration (MCHC),mean corpuscular volume (MCV),and serum iron (SI) were measured.ELISA was used to detect the duodenal divalent metal transporter (DMT-1) and membrane iron transfer protein transport (FPN-1).Results After 4 weeks of modeling,the RBC,HGB,HCT,MCH,MCHC and MCV in model group were significantly decreased (P <0.05).Compared with the model control group,the HGB,HCT,MCH,MCHC and MCV in the Ejiao Qianggu low-dose,medium-dose and high-dose groups were significantly increased (P<0.05);the SI and hemoglobin content and the DMT-1 protein in the medium-and high-dose Ejiao Qianggu oral liquid groups were significantly increased (P <0.01);and the protein content of FPN-1 in the high dose group was significantly increased (P <0.01).Conclusion Ejiao Qianggu oral liquid has a significant therapeutic effect on with rats iron deficiency anemia,by increasing both the hemoglobin content and the serum iron content.Its mechanism is closely related to intestinal DMT-1 and FPN-1.

关 键 词：阿胶强骨口服液 缺铁性贫血 血红蛋白 

分 类 号：R285[医药卫生—中药学]