中药千金黄连丸的抗动脉粥样硬化作用  

Anti-atherogenic effect of Chinese polyherbal Qianjin Huanglian Wan

作　　者：李静[1,2,3] 陈泫锦 佘成烨 邓远雄 LI Jing;CHEN Xuan-jin;SHE Cheng-ye;DENG Yuan-xiong(Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province,Hunan Normal University,Changsha 410013;Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Hunan Normal University),Ministry of Education,Changsha 410081;Department of Pharmaceutical Science,College of Medicine,Hunan Normal University,Changsha 410013)

机构地区：[1]湖南师范大学“小分子靶向药物研究与创制”湖南省重点实验室,长沙410013 [2]湖南师范大学“化学生物学及中药分析”教育部重点实验室,长沙410081 [3]湖南师范大学医学院药学系,长沙410013

出　　处：《中南药学》2021年第12期2569-2575,共7页Central South Pharmacy

基　　金：湖南省自然科学基金(No.2020JJ4437);湖南省教育厅重点项目(No.18A033);湖南师范大学“化学生物学及中药分析”教育部重点实验室开放基金(No.KLCBTCMR18-05);湖南师范大学“大学小分子靶向药物研究与创制”湖南省重点实验室开放基金(No.2019CG05);湖南省中医药管理局项目(No.2014104)。

摘　　要：目的探讨千金黄连丸(QHW)对动脉粥样硬化大鼠的影响及其初步机制。方法采用高效液相色谱法测定QHW提取物中小檗碱和梓醇的含量。采用高胆固醇饮食并给予过量维生素D;诱导大鼠动脉粥样硬化模型,研究QHW提取物对动脉粥样硬化大鼠血脂的影响以及对动脉粥样硬化大鼠主动脉壁的形态的影响。采用巨噬细胞诱导泡沫细胞模型,研究QHW提取物及含药血清对泡沫细胞形成的抑制作用,探讨QHW抗动脉粥样硬化的初步机制。结果 QHW提取物能显著调节血脂水平,与模型组比,高剂量组TC、TG、LDL-C水平显著下降(P<0.01),HDL-C水平显著升高(P<0.01)。QHW提取物可以显著改善动脉粥样硬化大鼠主动脉血管壁的形态特征。含药血清和QHW提取物均能降低泡沫细胞中胆固醇酯的含量,防止泡沫细胞形成。其中含药血清降低泡沫细胞中胆固醇酯含量的效果更好。结论 QHW对高胆固醇饮食诱导的动脉粥样硬化大鼠具有明显的抗动脉粥样硬化作用。QHW可能通过抑制泡沫细胞的形成和调节血脂发挥抗动脉粥样硬化的作用。Objective To study the effect of Qianjin Huanglian Wan (QHW) on atherosclerotic rats and its possible mechanism.Methods The content of berberine and catalpol in QHW extract was determined by HPLC.Atherosclerotic model rats induced by high cholesterol diet followed by administration of excessive vitamin D;were determined.The effect of QHW extract on the blood lipids of atherosclerotic rats was investigated,and the morphological changes of the aortic wall in the atherosclerotic rats were observed.The foam cell models were induced by macrophage.The inhibitory effect of QHW extract on the formation of foam cells was investigated and the anti-atherogenic mechanism of QHW explored.Results QHW extract significantly regulated the blood lipid level.Compared with the model group,the levels of TC,TG and LDL-C in the high-dose group of QHW decreased (P <0.01),and the level of HDL-C increased (P <0.01).The morphological characteristics of the aortic wall in atherosclerotic rats improved in QHW groups.Cell experiments showed that both the serum (from the rats treated with high dose of QHW extract) and QHW extract reduced the content of cholesteryl ester in the foam cells and prevented the formation of foam cells.The cholesteryl ester content in the foam cells reduced after treating with high dose of QHW extract.Conclusion QHW has anti-atherogenic effect on atherosclerotic rats induced by high-cholesterol diet induced.QHW exerts anti-atherogenic effect by inhibiting the formation of foam cells and regulating the blood lipids.

关 键 词：千金黄连丸 动脉粥样硬化 血脂 泡沫细胞 黄连 生地黄 

分 类 号：R285[医药卫生—中药学]