黄芪多糖调节维生素D/p38MAPK/ERK信号通路改善庆大霉素致肾小管上皮细胞损伤的机制  

The mechanism of Astragalus Polysaccharide regulating vitamin D/p38MAPK/ERK signaling pathway ameliorate gentamicin induced renal tubular epithelial cell injury

作　　者：吴雍真 李倩 管连城 李文 高洁 陈云志 朱星 柴艺汇 WU Yong-zhen;LI Qian;GUAN Lian-cheng;LI Wen;GAO Jie;CHEN Yun-zhi;ZHU Xing;CHAI Yi-Hui(Guizhou University of Traditional Chinese Medicine,Guiyang,550025 China;Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang,550002 China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州中医药大学第二附属医院,贵州贵阳550002

出　　处：《时珍国医国药》2021年第10期2312-2315,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81660762,81874376);贵州省中医药管理局中医药、民族医药科学技术研究专项(201920452);贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]221);贵州省国内一流建设学科(GNYL[2017]008号);贵阳中医学院研究生教育创新计划(YFYYJSCX2018-16号)。

摘　　要：目的探讨黄芪多糖(APS)改善庆大霉素(GM)致肾小管上皮细胞损伤的分子机制。方法MTT法检测正常对照组(加入等体积的细胞培养液)、模型组(GM 2mg·mL^(-1))、APS低剂量组(GM 2mg·mL^(-1)+APS 0.8mmol·L^(-1))、APS高剂量组(GM 2mg·mL^(-1)+APS 1.6mmol·L^(-1))、VD组(GM 2mg·mL^(-1)+VD 10^(-8) mol·L^(-1))肾小管上皮细胞存活率。Western blot检测细胞维生素D受体(VDR)、1α-羟化酶(CYP27B1)、24-羟化酶(CYP24A1)、p38MAPK、p-ERK1/2蛋白表达。结果与正常组比较,模型组肾小管上皮细胞存活率显著降低(P<0.01),且在24h、36h、48h时细胞存活率随着造模时间的延长而降低,VDR、CYP27B1蛋白表达量显著下降(P<0.01),CYP24A1、p38MAPK、p-ERK1/2蛋白表达量显著升高(P<0.01)对比模型组,APS高、低剂量组与VD组均能明显逆转上述指标。结论APS可能是通过调节VD的表达进而影响p38MAPK与ERK1/2信号通路改善庆大霉素致肾小管上皮细胞损伤。Objective To investigate the molecular mechanism of astragalus polysaccharide(APS)in improving renal tubular epithelial cell injury induced by gentamicin.Methods MTT method was used to detect the renal tubular epithelial cell survival rate in normal control group(GM 2mg·mL^(-1)),model group(GM 2mg·mL^(-1)),APS low-dose group(GM 2mg·mL^(-1)+APS 0.8mmol·L^(-1)),APS high-dose group(GM 2mg·mL^(-1)+APS 1.6mmol·L^(-1)),and VD group(GM 2mg·mL^(-1)+VD 10^(-8) mol·L^(-1)).Western blot was used to detect VDR,CYP27B1,CYP24A1,p38MAPK,and p-ERK1/2 protein expression.Results Compared with the normal group,the survival rate of renal tubular epithelial cells in the model group was significantly reduced(P<0.01),and at 24h,36h,and 48h,the cell survival rate decreased with the extension of the modeling time,and the expression of VDR and CYP27B1 protein decreased significantly(P<0.01),CYP24A1,p38MAPK,and p-ERK1/2 protein expression levels were significantly increased(P<0.01);Compared with the model group,the APS high and low dose groups and the VD group can significantly reverse the above indicators.Conclusion APS may improve the renal tubular epithelial cell injury induced by gentamicin by regulating the expression of VD and then affecting the p38MAPK and ERK1/2 signaling pathways.

关 键 词：黄芪多糖 维生素D 庆大霉素 肾小管上皮细胞 

分 类 号：R285.5[医药卫生—中药学]