半夏泻心汤对Hp阳性慢性萎缩性胃炎小鼠趋化因子CXCL9、CXCL10及炎性因子的影响  

Effects of Pinellia Xiexin Decoction on Chemokines CXCL9,CXCL10 and inflammatory factors in mice with Hp positive chronic atrophic gastritis

作　　者：李慧臻[1] 王天麟[1] 马佳乐 刘琳[1] 孔祥茹[1] LI Hui-zhen;WANG Tian-lin;MA Jia-le;LIU Lin;KONG Xiang-ru(Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin,300150,China)

机构地区：[1]天津中医药大学第二附属医院,天津300150

出　　处：《时珍国医国药》2021年第10期2316-2320,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81573889);天津卫健委中医药重点领域项目(2020007)。

摘　　要：目的研究半夏泻心汤对Hp阳性慢性萎缩性胃炎(Chronic atrophic gastritis,CAG)小鼠胃组织及血清中趋化因子CXCL9、CXCL10及炎性因子IL-17A、IL-6、IL-22的影响,揭示半夏泻心汤治疗Hp相关性胃炎的作用机制。方法将64只C57BL/6小鼠随机分为正常组10只、造模组54只。造模组予Hp菌液灌服、自由饮用MNNG溶液。8周后,随机抽取造模组4只小鼠检测是否造模成功。将已成模的50只Hp阳性CAG小鼠随机分为模型组、四联组、半夏泻心汤全方组、辛开组、苦降组各10只。正常组、模型组予生理盐水灌胃;四联组予四联杀菌药物灌胃;全方组予半夏泻心汤浓煎剂灌胃;辛开组予辛开汤浓煎剂灌胃;苦降组予苦降汤浓煎剂灌胃。ELISA检测小鼠趋化因子CXCL9、CXCL10及炎性因子IL-17A、IL-6、IL-22的表达。结果模型组小鼠可见胃黏膜层变薄,腺体数量减少、结构紊乱、排列形态扭曲,部分可见肠化、异型增生。药物干预后均不同程度的改善上述病理表现。与正常组对比,模型组小鼠胃组织及血清中CXCL-9、CXCL-10表达水平均升高(P<0.01)。与模型组对比,四联组、半夏泻心汤全方组干预后小鼠胃组织中CXCL-9、CXCL-10表达均下降(P<0.01或P<0.05),血清中仅四联组CXCL-10表达下降(P<0.01)。与正常组对比,模型组小鼠胃组织及血清中IL-17A、IL-6、IL-22表达水平均升高(P<0.01)。与模型组对比,四联组、半夏泻心汤全方组干预后小鼠胃组织及血清中IL-17A、IL-6、IL-22表达均下降(P<0.01或P<0.05)。辛开组、苦降组干预后小鼠胃组织及血清中CXCL-9、CXCL-10及IL-17A、IL-6、IL-22表达有不同程度的下降。结论Hp阳性CAG小鼠胃组织及血清中CXCL9、CXCL10表达均明显升高,半夏泻心汤及四联杀菌法可通过调控趋化因子CXCL9、CXCL10,降低炎性因子IL-17A、IL-6、IL-22表达,发挥治疗CAG的作用。Objective To study the effects of Pinellia Xiexin Decoction on chemokines CXCL9,CXCL10 and inflammatory factors IL-17 A,IL-6 and IL-22 in the gastric tissues and serum of mice with Hp-positive chronic atrophic gastritis,and to reveal the mechanism of Pinellia Xiexin Decoction in the treatment of Hp positive chronic atrophic gastritis.Methods The 64 C57 BL/6 mice were randomly divided into the normal group(10 mice)and the modeling group(54 mice).The model group was gavaged with HP bacteria solution and freely drank MNNG solution.After 8 weeks,4 mice were randomly selected from the modeling group to test whether the modeling was successful.Fifty Hp positive CAG mice were randomly divided into model group,quadruple groups,Pinellia Xiexin whole group,Xinkai group and Kujiang group,each with 10 mice.Normal group and model group were given normal saline intragastric gavage.The quaternary group was given quaternary bactericidal drugs by gavage.The whole group was given Banxia Xiexin Decoction by intragastric administration;Xinkai group was given Xinkai decoction by gavage;Kujiang group was given Kujiang decoction by gavage.The expressions of chemokines CXCL9,CXCL10 and inflammatory factors IL-17 A,IL-6 and IL-22 were detected by ELISA.Results In the model group,the gastric mucosa was thinned,the number of glands was reduced,the structure was disordered,the arrangement was distorted,and some of them showed intestinal metaplasia and dysplasia.After drug intervention,the above pathological manifestations were improved in different degrees.Compared with normal group,the expression levels of CXCL-9 and CXCL-10 in gastric tissue and serum of mice in model group were increased(P<0.01).Compared with the model group,the expressions of CXCL-9 and CXCL-10 in the stomach tissues of mice in the quadruple group and the Pinellia Xiexin whole group were decreased after intervention(P<0.01 or P<0.05),and the expression of CXCL-10 in serum was decreased only in the quadruple group(P<0.01).Compared with normal group,the expression levels

关 键 词：半夏泻心汤 幽门螺杆菌相关性胃炎 趋化因子 CXCL9 CXCL10 炎性因子 

分 类 号：R285.5[医药卫生—中药学]