清肺平喘颗粒对哮喘模型大鼠气道重塑及TGF-β1/Smad信号通路的影响  

The effects of Qingfei Pingchuan Granule on airway remodeling and TGF-β1/Smad signaling pathway in asthmatic rats model

作　　者：迟映雪 石绍顺[1] CHI Ying-xue;SHI Shao-shun(Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang Liaoning 110034,China)

机构地区：[1]辽宁中医药大学附属第二医院,辽宁沈阳110034

出　　处：《时珍国医国药》2021年第10期2384-2387,共4页Lishizhen Medicine and Materia Medica Research

基　　金：辽宁省科技厅博士启动基金项目(20141165)。

摘　　要：目的观察清肺平喘颗粒对哮喘模型大鼠气道重塑及转化生长因子-βl(TGF-βl)/信号转导蛋白(Smad)信号通路的影响,探讨其治疗哮喘的作用机制。方法72只Sprauge-Dawley大鼠,随机分为清肺平喘颗粒低剂量组(A1)、清肺平喘颗粒中剂量组(A2)、清肺平喘颗粒高剂量组(A3)、地塞米松组(B)、模型对照组(C)、空白对照组(D),除D组外其它各组用卵蛋白(OVA)建立大鼠哮喘模型,造模成功后A1组给予含生药量0.315g清肺平喘颗粒(1ml)灌胃,A2组给予含生药量0.63g清肺平喘颗粒(1ml)灌胃,A3组给予含生药量1.26g清肺平喘颗粒(1ml)灌胃,B组给予含生药量0.09mg醋酸地塞米松溶液(1ml)灌胃,C、D组给予1ml生理盐水灌胃,每天一次,连续8周。观察各组大鼠肺组织病理学表现;测算支气管内管壁(横断面)面积(WAi),平滑肌(横断面)面积(WAm),基底膜周长(Pbm),计算WAi/Pbm、WAm/Pbm评估管壁及平滑肌厚度变化,蛋白印记分析(Western-blot)检测哮喘大鼠肺组织TGF-β1/Smad3、Smad7蛋白表达水平。结果与D组比较,C组WAi/Pbm、WAm/Pbm明显增加(P<0.01),与C组比较,A1、A2、A3、B组WAi/Pbm、WAm/Pbm明显较少(P<0.01);C组与D组比较TGF-βl、Smad3相蛋白达明显升高,Smad7蛋白表达明显降低(P<0.01),A2、A3、B组与C组比较TGF-βl、Smad3蛋白表达明显减低,Smad7蛋白表达明显升高(P<0.01),A2、A3、B组间比较没有显著性差异(P>0.05),A1组与C组比较没有显著性差异(P>0.05)。结论清肺平喘颗粒可改善哮喘模型大鼠气道重塑状态,其机制可能与调节TGF-β1/Smad信号通路有关。Objective To observe the effects of Qingfei Pingchuan Granule on airway remodeling and transforming growth factor-β1(TGF-β1)/Smad signaling pathway in asthmatic rats,and to explore the mechanism of Qingfei Pingchuan Granule in treating asthma.Methods Seventy two Sprague Dawley rats were randomly divided into Qingfei Pingchuan Granule low-dose group(A1),Qingfei Pingchuan Granule middle dose group(A2),Qingfei Pingchuan Granule high-dose group(A3),dexamethasone group(B),model control group(C)and blank control group(D).Except group D,in the other groups,asthma models were established using ovalbumin(OVA).After successful modeling,group A1 was given Qingfei Pingchuan Granule(1 ml)containing 0.315 g crude drug,group A2 was given Qingfei Pingchuan Granule(1 ml)containing 0.63 g crude drug,group A3 was given Qingfei Pingchuan Granule(1 ml)containing 1.26 g crude drug,group B was given 0.09 mg dexamethasone acetate solution(1 ml),group C and D were given 1 ml normal saline,once a day for 8 weeks.The pathological changes of lung tissue were observed;the area of bronchial wall(WAI),smooth muscle(WAM)and the perimeter of basement membrane(PBM)were measured.Wai/PBM and WAM/PBM were calculated to evaluate the thickness changes of tube wall and smooth muscle.The protein expression levels of TGF-β1/Smad3 and Smad7 in lung tissue of asthmatic rats were detected by Western blot.Results Compared with group D,Wai/PBM and WAM/PBM in group C were significantly increased(P<0.01),Compared with group C,Wai/PBM and WAM/PBM in groups A1,A2,A3 and B were significantly less(P<0.01);Compared with group D,TGF-β1 and Smad3 protein were significantly increased,and Smad7 protein expression was significantly decreased in group C(P<0.01),there was no significant difference between groups A2,A3 and B(P>0.05),and there was no significant difference between group A1 and group C(P>0.05).Conclusion Qingfei Pingchuan Granule can improve airway remodeling in asthmatic rats,and its mechanism may be related to the regulation of TGF-β1/Smad signaling pa

关 键 词：清肺平喘颗粒 哮喘 大鼠 气道重塑 TGF-Β1/SMAD信号通路 

分 类 号：R285.5[医药卫生—中药学]