固相萃取-高效液相色谱法测定烟叶中仲丁灵残留  

Determination of Butralin Residues in Tobacco Leaves by Solid Phase Extraction-High Performance Liquid Chromatography

作　　者：石妍[1] 肖顺[1] 周挺[2] 梁颁捷 张珊珊[3] 张绍升[1] 刘国坤[1] 顾钢[2] SHI Yan;XIAO Shun;ZHOU Ting;LIANG Ban-jie;ZHANG Shan-shan;ZHANG Shao-sheng;LIU Guo-kun;GU Gang(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Tobacco Science,Fujian Tobacco Company,Fuzhou 350003,China;Sanming Branch of Fujian Tobacco Company,Sanming 365000,Fujian,China)

机构地区：[1]福建农林大学植物保护学院,福州350002 [2]福建省烟草公司烟草科学研究所,福州350003 [3]福建省烟草公司三明市公司,福建三明365000

出　　处：《农药》2021年第12期900-903,共4页Agrochemicals

基　　金：中国烟草总公司福建省公司科技项目,闽烟司科(201635000020073,201735000027131)号。

摘　　要：[目的]建立固相萃取-高效液相色谱法测定烟叶中仲丁灵残留量的方法。[方法]样品用乙腈提取、NaCl盐析、Florisil小柱净化,以乙腈-水(体积比75:25)为流动相,经C_(18)液相色谱柱分离,二极管阵列检测器检测,检测波长235 nm。[结果]仲丁灵在质量浓度为0.2~10.0 mg/L范围内线性关系良好(R^(2)=0.9996),检出限为0.025 mg/kg。在0.5、1.0、5.0 mg/kg添加水平下的加标回收率为80.04%~95.95%,相对标准偏差(RSD)为1.37%~8.67%。[结论]该方法操作简单、快速、准确,可用于烟叶中仲丁灵残留检测。[Aims]This study aims to develop a method for the determination of butralin residues in tobacco leaves by solid phase extraction-high performance liquid chromatography(HPLC).[Methods]The samples were extracted with acetonitrile,and salted out with NaCl,purified with Florisil column;then separated on a C_(18) chromatographic column with acetonitrile and water(75:25,by vol)as the mobile phase and detected by photo-diode array(PDA)at 235 nm.[Results]The butralin had fine linear relationships in the range of 0.2-10.0 mg/L(R^(2)=0.9996),and the limit of detection for tobacco leaves was 0.025 mg/kg.The recoveries were 80.04-95.95%,with the relative standard deviations of 1.37-8.67%at the three different spiked levels of 0.5,1.0 and 5.0 mg/kg.[Conclusions]This method is simple,fast and accurate.It is suitable for the determination of butralin residues in tobacco leaves.

关 键 词：仲丁灵 固相萃取 高效液相色谱 烟叶 

分 类 号：TQ450.2[化学工程—农药化工]