Boronic acid-containing carbon dots array for sensitive identification of glycoproteins and cancer cells  

作　　者：Shuai Chen Xuewei Zhang Yongliang Yu Jianhua Wang 

机构地区：[1]College of Life and Health Sciences,Northeastern University,Shenyang 110169,China [2]Research Center for Analytical Sciences,Department of Chemistry,College of Sciences,Northeastern University,Shenyang 110819,China

出　　处：《Chinese Chemical Letters》2021年第10期3043-3047,共5页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21922402,21874017 and21727811);Liaoning Provincial Program for Promoting Talents(Nos.XLYC1807005 and XLYC1802016);the Key Laboratory of Data Analytics and Optimization for Smart Industry(Northeastern University,Ministry of Education,China,111 Project,No.B16009)。

摘　　要：Discrimination of glycoproteins and cell types is a significant but difficult issue.Herein,we presented a novel fluorescence sensor array for the detection and identification of glycoproteins and cancer cells based on the specific affinity between boronic acid-containing carbon dots(BA-CDs)and cis-diol residues of polysaccharides.The differential binding affinity of three BA-CDs to various glycoproteins resulted in a different fluorescence turn-on signal pattern caused by aggregation-enhanced emission(AEE),along with negligible response from other proteins.Therefore,BA-CDs encompassing sensing elements and signal indicator into one can enable a fast and accurate discrimination of glycoproteins with simple and easy operation.Seven glycoproteins could be well discriminated at a very low concentration of 10 nmol/L.The discriminating capability of glycoproteins is not sacrificed in both human urine and serum.Notably,different glycoprotein compositions of cancer cells provide more recognizable features for identification of cancer cells,comparing to the total protein.Five cell types could be identified in 15 min at a low concentration of 1000 cells/mL.This method is fast,accurate,and easy operation,and has a potential application in cancer diagnosis.

关 键 词：Sensor array Boronic acid Carbon dots Glycoproteins and cells Aggregation-enhanced emission 

分 类 号：O657.3[理学—分析化学] R730.4[理学—化学]