西伯利亚杏开花相关基因的转录组测序分析  

Transcriptome Sequencing Analysis of Flowering Related Genes in Prunus sibirica

作　　者：陈晨 徐宛玉 朱高浦[1] 赵罕[1] 刘慧敏[1] 王淋[1] 乌云塔娜[1] Chen Chen;Xu Wanyu;Zhu Gaopu;Zhao Han;Liu Huimin;Wang Lin;Wuyun Tana(Key Laboratory of Non-timber Forest Germplasm Enhancement and Utilization of National Forestry and Grassland Administration,Paulownia Research and Development Center of China,National Forestry and Grassland Administration,Non-timber Forest Research and Development Center,Chinese Academy of Forestry,Zhengzhou,450003)

机构地区：[1]中国林业科学研究院,经济林研究开发中心,国家林业和草原局泡桐研究开发中心,经济林种质创新与利用国家林业和草原局重点实验室,郑州450003

出　　处：《分子植物育种》2021年第22期7422-7430,共9页Molecular Plant Breeding

基　　金：河南省重点研发与推广专项(192102110063);国家重点研发计划项目(2018YFD1000606-3)。

摘　　要：为探明调控西伯利亚杏(Prunus sibirica)成花的分子机制,筛选参与调控开花的相关候选基因,本研究以两个不同时期的西伯利亚杏花芽为样本进行转录组测序,共获得42.04 Gb高质量数据,平均95.6%的数据均可以比对上参考基因组。共筛选出6850个显著差异表达基因,其中在花芽萌动期上调表达基因有2784个,下调表达基因4066个,花芽萌动期特异表达基因392个,盛花期特异表达基因346个。KEGG富集结果表明差异表达基因在植物激素信号转导、苯丙素生物合成、淀粉和蔗糖代谢等代谢通路被富集最多。另外,本研究共发现39个显著差异表达基因涉及植物开花调控的相关途径,主要包括:春花途径相关基因中10个、光周期途径相关基因17个、自主途径相关基因5个、霉素途径相关基因1个、温度途径相关基因2个,整合因子(SOC1,FT,LFY)共4个。研究结果可为西伯利亚杏开花调控提供了重要的候选基因,对西伯利亚杏育种具有一定的参考价值。In order to explore the molecular mechanism of regulating flowering in Prunus sibirica,the transcriptome of flower buds at two different stages was sequenced to investigate the candidate genes involved in the flowering.A total of 42.04 Gb clean data were obtained,and more than 92.00% of the clean reads were mapped to the reference genome.A total of 6 850 significantly differentially expressed genes(DEGs) were found,including 2 784 significantly up-regulated and 4 066 significantly down regulated genes.We further found 392 and 346 DEGs were special in sprouting stage and full-bloom stage,respectively.KEGG enrichment analysis showed that the first three DEGs were enriched in plant hormone signal transduction,phenylpropanoid biosynthesis,and starch and sucrose metabolism,respectively.Among these DEGs,39 genes involved in the flowering regulation pathways were obtained,including 10,17,5,1,and 2 genes in vernalization pathway,photoperiod pathway,autonomous pathway,gibberellin pathway,and temperature pathway,respectively.In addition,four integrators,including SOC1,FT,and LFY,were also identified.This study will provide the valuable information for studying the candidate genes involved in the flowering and for breeding in P.sibirica.

关 键 词：西伯利亚杏 开花调控 差异表达基因 转录组 

分 类 号：S662.2[农业科学—果树学]