苦豆子总碱降血糖作用及其机制研究  

Hypoglycemic effect and mechanism of total alkaloids of Sophora alopecuroides L.

作　　者：刘伟 张扬星 杨新洲[2] 欧阳艳 赵平[2] LIU Wei;ZHANG Yang-xing;YANG Xin-zhou;OUYANG Yan;ZHAO Ping(Key Lab of Natural Product Chemistry and Application at Universities of Education Department of Xinjiang Uygur Autonomous Region,Yili Normal University,Yining 835000,China;South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]伊犁师范大学新疆维吾尔自治区教育厅高校天然产物化学与应用重点实验室,伊宁835000 [2]中南民族大学,武汉430074

出　　处：《天然产物研究与开发》2021年第12期1991-1997,共7页Natural Product Research and Development

基　　金：新疆维吾尔自治区自然科学基金(2019D01C335);中南民族大学中央高校基本科研业务费专项(CZY20004);武陵山区特色资源植物物种保护与利用湖北省重点实验室项目(2018BFC360)。

摘　　要：本文探究苦豆子地上部分提取的苦豆子总碱对L6骨骼肌细胞降血糖的分子机制。利用葡萄糖检测试剂盒发现苦豆子总碱(total alkaloids of Sophora alopecuroides L.,TASA)能增加L6细胞的葡萄糖摄取,且MTT结果显示TASA对L6细胞没有药物毒性;利用激光共聚焦显微镜监测到TASA能促进GLUT4的转位;Western blot结果显示TASA对L6细胞内GLUT4的表达有一定促进作用,且能增强AMPK和PKC的磷酸化水平。这些结果表明TASA通过AMPK和PKC两种信号通路来促进GLUT4的表达和转运继而促进葡萄糖的摄取,说明TASA可能具有开发成新型降糖药的潜力。The aim of this study is to explore the molecular mechanism of the total alkaloids of Sophora alopecuroides(TASA)extracted from the aboveground part of S.alopecuroides on the hypoglycemic effect of L6 skeletal muscle cells.TASA was found to increase glucose uptake in L6 cells using glucose detection kit,and MTT results showed that TASA had no cytotoxicity on L6 cells.TASA could promote the translocation of GLUT4 by laser confocal microscopy.Western blot results showed that TASA could promote the expression of GLUT4 in L6 cells and enhanced the phosphorylation of AMPK and PKC.These results suggested that TASA could promote the expression and transport of GLUT4 through AMPK and PKC signaling pathways,and then promote glucose uptake,suggesting that TASA may have the potential to be developed as a novel hypoglycemic drug.

关 键 词：苦豆子总碱 2型糖尿病 L6骨骼肌细胞 葡萄糖转运蛋白4 信号通路 

分 类 号：R285.5[医药卫生—中药学]