丁香茄叶乙酸乙酯部位化学成分及抗炎活性研究  

Chemical constituents from the EtOAc extract of Calonyction muricatum leaves and their anti-inflammatory activity

作　　者：白岩松 池军 张玲霞[1,3,4] 冯庆梅 代丽萍 王智民[3,4,5] BAI Yan-song;CHI Jun;ZHANG Ling-xia;FENG Qing-mei;DAI Li-ping;WANG Zhi-min(School of Pharmacy,Henan University of Chinese Medicine;Academy of Chinese Medical Sciences,Henan University of Chinese Medicine;Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province;Zhengzhou Engineering Research Center for Food-Medicine Homologous Substances from Henan Province,Zhengzhou 450046,China;Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]河南中医药大学药学院 [2]河南中医药大学中医药科学院 [3]河南省豫产道地药材综合开发利用工程技术研究中心 [4]郑州市豫产食药物质研发工程研究中心,郑州450046 [5]中国中医科学院中药研究所,北京100700

出　　处：《天然产物研究与开发》2021年第12期2011-2018,共8页Natural Product Research and Development

基　　金：国家重点研发计划(2017YFC1701904-3)。

摘　　要：基于脂多糖(LPS)诱导的小鼠巨噬细胞(RAW 264.7)生物模型研究丁香茄Calonyction muricatum叶乙酸乙酯部位的抗炎活性成分。在体外活性追踪下,采用硅胶、MCI、ODS、HW-40C柱色谱及半制备液相等方法对乙酸乙酯活性部位进行分离纯化,根据理化性质结合现代波谱技术鉴定化合物结构。从丁香茄叶乙酸乙酯部位分离得到15个化合物,分别为:12-oxo-phytodienoic acid(1)、(9 Z,12 Z,14 E,16 R)-16-hydroxyoctadeca-9,12,14-trienoic acid(2)、(9 Z,12 S,13 E,15 Z)-12-hydroxyoctadeca-9,13,15-trienoic acid methyl ester(3)、(9 Z,11 E,13 R,15 Z)-13-hydroxyoctadeca-9,11,15-trienoic acid(4)、(9 R,10 E,12 Z)-9-hydroxyoctadeca-10,12-dienoic acid(5)、(9 S,10 E,12 Z)-9-hydroxyoctadeca-10,12-dienoic acid(6)、1-十二烷醇(7)、山柰酚-3-O-半乳糖苷(8)、6-methoxykaempferol-3-O-galactoside(9)、紫云英苷(10)、6-methoxykaempferol-3-O-glucoside(11)、(6 S,9 R)-长寿花糖苷(12)、咖啡酸乙酯(13)、麦芽酚(14)、hydroxydihydrobovolide(15)。化合物1~12、14、15为首次从月光花属植物中分离得到。单体化合物抗炎活性筛选结果表明,化合物1、3、4、5均具有一定的抗炎活性,其中1和5的抑制NO作用显著,IC_(50)值分别为1.17±0.51、0.97±0.89μM。To study the anti-inflammatory constituents from the EtOAc extract of Calonyction muricatum leaves based on lipopolysaccharide(LPS)-induced mouse macrophages(RAW 264.7)as the cell model in vitro.Under the guidance of bioactivity screening,the phytochemistry investigation of active EtOAc extract was carried out by silica gel,MCI,ODS,HW-40C column chromatography and semi-prep HPLC methods.The structures of isolated compounds were identified on the basis of physicochemical properties and modern spectroscopy technologies.A total of 15 compounds were obtained from C.muricatum leaves,including 12-oxo-phytodienoic acid(1),(9 Z,12 Z,14 E,16 R)-16-hydroxyoctadeca-9,12,14-trienoic acid(2),(9 Z,12 S,13 E,15 Z)-12-hydroxyoctadeca-9,13,15-trienoic acid methyl ester(3),(9 Z,11 E,13 R,15 Z)-13-hydroxyoctadeca-9,11,15-trienoic acid(4),(9 R,10 E,12 Z)-9-hydroxyoctadeca-10,12-dienoic acid(5),(9 S,10 E,12 Z)-9-hydroxyoctadeca-10,12-dienoic acid(6),1-dodecanol(7),kaempferol-3-O-galactoside(8),6-methoxykaempferol-3-O-galactoside(9),astragaline(10),6-methoxykaempferol-3-O-glucoside(11),(6 S,9 R)-roseoside(12),ethyl caffeate(13),maltol(14)and hydroxydihydrobovolide(15).Among them,compounds 1-12,14 and 15 were isolated from the genus Calonyction for the first time.Additionally,the bioassay results showed that compounds 1,3,4 and 5 all had certain anti-inflammatory actions.Compounds 1 and 5 significantly inhibited NO production with IC_(50) values of 1.17±0.51 and 0.97±0.89μM respectively.

关 键 词：丁香茄叶 乙酸乙酯部位 十八碳不饱和脂肪酸 黄酮苷 抗炎活性 

分 类 号：R284.2[医药卫生—中药学]