蒲公英根多糖硫酸酯化工艺条件优化及其对HepaRG细胞氧化损伤的保护作用  

Optimization of sulfation conditions of dandelion root polysaccharide and its protective effect on oxidative damage of human HepaRG cells

作　　者：朱永红[1] 范震宇[1] 韩勇 吴雯仪 蔡亮亮 ZHU Yong-hong;FAN Zhen-yu;HAN Yong;WU Wen-yi;CAI Liang-liang(Department of Pharmacy,Affiliated Hospital of Nantong University;Oncology of Department,Affiliated Hospital of Nantong University;Sinopharm holding Nantong Co.,Ltd.,Nantong 226001,China)

机构地区：[1]南通大学附属医院药学部 [2]南通大学附属医院肿瘤科 [3]国药控股南通有限公司,南通226001

出　　处：《天然产物研究与开发》2021年第12期2082-2089,共8页Natural Product Research and Development

基　　金：国家自然科学基计划(81900528);江苏省博士后科研资助计划(2020Z435);南通市药学会-常州四药医院药学科研基金(ntyx2004)。

摘　　要：本研究采用响应曲面法、MTT比色法分别研究蒲公英根多糖的硫酸酯化工艺与蒲公英根多糖及其硫酸酯对H_(2)O_(2)诱导的HepaRG细胞氧化损伤的保护作用。结果表明:蒲公英根多糖硫酸酯化反应的最优条件为反应温度83℃、吡啶与氯磺酸比为8、反应时间3 h。蒲公英根多糖及其硫酸酯对H_(2)O_(2)诱导的HepaRG细胞氧化损伤均具有保护作用,而且硫酸酯化蒲公英根多糖的活性优于蒲公英根多糖。In this study,response surface methodology(RSM)and MTT colorimetry were used to study the sulfation process of dandelion root polysaccharide and the protective effects of dandelion root polysaccharide and its sulfation on oxidative damage of HepaRG cells induced by hydrogen peroxide(H_(2)O_(2)).The results showed that the optimum conditions for sulfation of dandelion root polysaccharides were as follows:reaction temperature was 83℃,ratio of pyridine to chlorosulfonic acid was 8,reaction time was 3 h.Moreover,dandelion root polysaccharide and its sulfates had protective effects on H_(2)O_(2)induced oxidative damage of HepaRG cells,and the activity of sulfated dandelion root polysaccharide was better than that of dandelion root polysaccharide.

关 键 词：蒲公英根多糖 响应曲面法 硫酸酯 氧化损伤 

分 类 号：R284.3[医药卫生—中药学]