基于智能手机图片比色法对化妆品中甾体激素含量的快速检测  

Rapid Quantitative Detection of Steroid Hormones in Cosmetics Based on Smart Phone Image Colorimetry

作　　者：刘红 王林 杨飘飘 李丽霞 陈磊[3] LIU Hong;WANG Lin;YANG Piaopiao;LI Lixia;CHEN Lei(Hubei Institute for Drug Control,Wuhan 430075,Hubei,China;Hubei Engineering Research Center for Drug Quality Control,Wuhan 430075,Hubei,China;School of Computer Science,Wuhan University,Wuhan 430072,Hubei,China)

机构地区：[1]湖北省药品监督检验研究院,湖北武汉430075 [2]湖北省药品质量检测与控制工程技术研究中心,湖北武汉430075 [3]武汉大学计算机学院,湖北武汉430072

出　　处：《香料香精化妆品》2021年第6期1-4,共4页Flavour Fragrance Cosmetics

基　　金：湖北省药品监督管理局药品安全监管项目(编号:20210105)。

摘　　要：提出了基于智能手机图片比色法实现甾体激素含量快速检测的方法,对显色条件、显色稳定性、拍摄距离等因素对实验的影响进行了考察和优化。在优化条件下,测定的相关系数良好,大于0.99,检出限为0.02 mg,3个质量浓度水平下检测结果重复性的相对标准偏差(RSD)分别为1.8%、1.5%和5.5%;并考察了3款不同型号智能手机检测的普适性,结果表明,3款手机均可作为采集设备,检测效果与手机型号无明显关联。采用智能手机图片比色法与分光光度法对样品检测的结果基本一致,表明智能手机图片比色法适用于甾体激素含量的快速测定。A new method for rapid quantitative detection of steroid hormones based on smart phone image colorimetry was proposed,and the influence of color development conditions,color stability,shooting distance and other factors on the experiment were investigated and optimized.Under optimal conditions,the correlation coefficient of the determination was good and greater than 0.99,the detection limit was 0.02 mg,and the relative standard deviations(RSD)of the repeatability of three mass concentration levels were 1.8%,1.5%,and 5.5%,respectively.The universality of the detection by three different types of smart phones was investigated,and the results showed that all of three types of smart phones could be used as acquisition equipments for the determination,and the detection results were not significantly related to the phone models.The results obtained by the smart phone image colorimetry were basically consistent with those by the spectrophotometry,indicating that the smart phone image colorimetry was suitable for the rapid determination of steroid hormones in cosmetics.

关 键 词：图片比色法 智能手机 甾体激素 丙酸氯倍他索 

分 类 号：TQ658[化学工程—精细化工]