大连地区分泌型与非分泌型母亲母乳菌群结构的差异  

Differences in structure of microbial community in breast milk between secretory and non-secretory mothers in Dalian

作　　者：罗迪[1,2] 董建一 李明 袁杰力[2] 刘银辉[2] LUO Di;DONG Jian-yi;LI Ming;YUAN Jie-li;LIU Yin-hui(Clinical Laboratory,the Second People′s Hospital of Dalian,Dalian,Liaoning 116011,China;Dalian Medical University,Dalian,Liaoning 116044,China;不详)

机构地区：[1]大连市第二人民医院检验科,辽宁116011 [2]大连医科大学微生态学教研室 [3]大连医科大学实验动物中心

出　　处：《中国微生态学杂志》2021年第11期1249-1253,共5页Chinese Journal of Microecology

基　　金：国家自然科学基金(31900920);大连市科技创新基金(2020JJ27SN068);广州合生元营养护理研究院科研基金(2019BINCMCF02)。

摘　　要：目的探讨大连地区分泌型和非分泌型母亲哺乳期间母乳菌群的主要差异。方法于大连市妇幼保健院纳入42名志愿者产妇,收集其产后第6天的母乳样本。提取母乳样本DNA,并对包含rs601338和rs1047781单核酸位点多态性(SNP)的片段用聚合酶链式反应(polymerase chain reaction,PCR)检测并加以测序,以检测受试母亲岩藻糖基转移酶2基因(FUT2)的类型。采用16SrRNA高通量测序法对不同母乳样本中微生物的多样性进行分析,并且对不同母乳样本中的微生物丰度及类型展开探讨。结果母亲分泌型分布情况分析:42名志愿者中,36名母亲是分泌型,6名母亲是非分泌型。母乳菌群多样性分析:分泌型与非分泌型组母亲母乳菌群比较,发现反映组内差异的Alpha多样性指数(包括Ace、Chao1以及Shannon等)在2组间差异无统计学意义;通过使用主坐标分析,发现分泌型组和非分泌型组的距离相对较远,母乳菌群组内菌群结构类似,组间差异性较显著,说明母乳中的蛋白核心岩藻糖基化水平可明显改变母乳中菌群结构。乳汁菌群物种构成分析:从门水平分析,变形菌门与厚壁菌门为主要优势菌;从属水平分析,2组母乳菌群中的双歧杆菌属丰度差异较显著。相关性分析:分泌型母乳的菌群中相关基因的表达更强,说明分泌型母乳对菌群基因的表达有促进作用。结论母乳菌群构成在一定程度上受到母体FUT2基因类型,即母亲分泌型和非分泌型的影响。Objective To observe main differences in breast milk microbiota between secretory and non-secretory mothers during breastfeeding in Dalian,Liaoning province.Methods A total of 42volunteer mothers in Dalian Maternity Hospital were enrolled.The samples of their breast milk on the 6th day after delivery were collected.The DNAs of the breast milk samples were extracted,and the fragments containing SNP were detected with Polymerase Chain Reaction(PCR)and sequenced to determine the gene type of FUT2.High throughput sequencing of 16SrRNA was used to analyze the microbial diversity,and the microbial abundance and types in different breast milk samples were discussed.Results Among the 42mothers,36 were secretory type and 6were non-secretory type.Systematic analysis showed that there were no significant differences inαdiversity indexes such as Shannon,Ace and Chao1between the two groups.Principal coordinate analysis showed that the distance between secretory type and non-secretory type was relatively long.The structure of microflora in lactobacillus group was similar,while there were great differences between groups,suggesting that the level of core fucosylation of breast milk protein could significantly change the structure of microflora in breast milk.At the phylum level,Proteobacteria and Firmicutes were the main dominant bacteria.At the genus level,there was a significant difference in the abundance of Bifidobacteriumbetween the two groups.The expressions of related genes in the microflora from secretory breast milk were stronger,indicating that secretory breast milk could promote the expression of microflora genes.Conclusion The composition of breast milk microbiota is affected by maternal FUT2gene types,namely maternal secretory and non-secretory.

关 键 词：母乳分型 FUT2基因 母乳菌群 

分 类 号：R378[医药卫生—病原生物学]