7-羟乙基白杨素对低压性缺氧大鼠运动性疲劳具有保护作用  

Protective effects of 7-hydroxyethyl chrysin on rats with exercise-induced fatigue in hypobaric hypoxia environment

作　　者：苗路伟 赵彤 高迎春 景临林 黄琼 马慧萍 MIAO Luwei;ZHAO Tong;GAO Yingchun;JING Linlin;HUANG Qiong;MA Huiping(College of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Key Laboratory of the Plateau Medicine,the 940th Hospital of Joint Logistics Support Force of Chinese Peopled Liberation Army,Lanzhou 730050,China;Medical Support Center,the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]中国人民解放军联勤保障部队第九四〇医院药剂科全军高原医学实验室,甘肃兰州730050 [3]中国人民解放军联勤保障部队第九四0医院医疗保障中心,甘肃兰州730050

出　　处：《浙江大学学报（医学版）》2021年第5期575-581,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(81571847,81872796);军队后勤科研计划(CWH17J010,CLB18J028)。

摘　　要：目的:探究7-羟乙基白杨素(7-HEC)对低压性缺氧大鼠运动性疲劳的保护作用。方法:选取40只健康雄性Wistar大鼠随机分为常氧对照组、缺氧对照组、白杨素组、7-HEC组,每组各10只,白杨素组和7-HEC组分别给予白杨素和7-HEC350 mg/kg,常氧对照组和缺氧对照组给予等量灭菌注射用水。常氧对照组饲养于当地海拔;其余三组置于低压性缺氧动物实验舱模拟7000 m海拔进行缺氧处理。连续灌胃给药3 d后进行力竭实验,统计各组游泳时间。力竭实验后立即采集大鼠的血液、肝组织和骨骼肌组织,观察肝组织和骨骼肌组织病理学变化,测定血尿素氮、乳酸、丙二醛、总超氧化物歧化酶(T-SOD)、肌肝糖原含量等指标。结果:与缺氧对照组比较,7-HEC组负重游泳时间延长[(2.58±0.77)和(4.04±1.30)min,P<0.01];肝组织和骨骼肌组织的病理学变化改善;血尿素氮[(3.24±0.93)和(2.22±0.60)mmol·L–1·min–1]、乳酸[肝组织为(0.14±0.05)和(0.10±0.03)mg·g–1·min–1,骨骼肌组织为(0.26±0.06)和(0.18±0.07)mg·g–1·min–1]、丙二醛[肝组织为(1.37±0.48)和(0.78±0.28)nmol·mg–1·min–1,骨骼肌组织为(0.87±0.19)和(0.63±0.11)nmol·mg–1·min–1]生成速率减小(均P<0.05);糖原[肝组织为(15.16±2.69)和(18.89±2.88)mg/g,骨骼肌组织为(1.46±0.49)和(1.78±0.48)mg/g]和T-SOD[肝组织为(1.87±0.01)和(2.68±0.12)U/mL,骨骼肌组织为(5.46±0.42)和(6.52±0.96)U/mL]增加(均P<0.05)。结论:7-HEC对低压性缺氧大鼠运动性疲劳具有明显保护作用。Objective:To investigate the protective effect of 7-hydroxyethyl chrysin(7-HEC)on rats with exercise-induced fatigue in hypobaric hypoxic condition.Methods:Forty healthy male Wistar rats were randomly divided into four groups with 10 rats in each group:control group,model group,chrysin group and 7-HEC group.The rats in control group were raised at local altitude but other three groups were raised in a simulating altitude of 7000 m for hypobaric hypoxia treatment.The chrysin group and 7-HEC group were given 350 mg/kg chrysin or 7-HEC by gavage for 3 d,respectively;while the control group and model group were given the same amount of sterilized water.The weight-bearing swimming tests were performed 3 d later,and the weight-bearing swimming time was documented.After rats were sacrificed,the liver and skeletal muscle tissue samples were taken for pathological examination and determination of lactate,malondialdehyde(MDA),total superoxide dismutase(T-SOD)and glycogen levels.Blood urea nitrogen was also determined.Results:Compared with the model group,weight-bearing swimming times were significandy prolonged in 7-HEC group[(2.58±0.77)vs.(4.04±1.30)min,P<0.01];pathological changes in liver and skeletal muscle tissue were attenuated;generation rate of blood urea nitrogen[(3.24±0.93)vs.(2.22±0.60)mmol·L^(-1)·min^(-1),P<0.05],lactate[liver:(0.14±0.05)vs.(0.10±0.03)mg·g^(-1)·min^(-1)skeletal muscle:(0.26±0.06)vs.(0.18±0.07)mg·g^(-1)·min^(-1)and MDA liver:(1.37±0.48)vs.(0.78±0.28)nmol·mg^(-1)·min^(-1),skeletal muscle:(0.87±0.19)vs.(0.63±0.11)nmol·L^(-1)·min^(-1)]were significantly reduced(all P<0.05);glycogen content[liver:(15.16±2.69)vs.(18.89±2.88)mg/g,skeletal muscle:(1.46±0.49)vs.(1.78±0.48)mg/g]and T-SOD[liver:(1.87±0.01)vs.(2.68±0.12)U/mL,skeletal muscle:(5.46±0.42)vs.(6.52±0.96)U/mL]were significantly improved(all P<0.05).Conclusion:7-HEC has significant protective effect on the rats with exercise-induced fatigue in hypobaric hypoxia condition.

关 键 词：7-羟乙基白杨素 抗疲劳 低压性缺氧 力竭实验 大鼠 

分 类 号：R965.[医药卫生—药理学]