Hirsutanol A对脂多糖诱导的A549细胞损伤的影响  

Effect of Hirsutanol A on lipopolysaccharide-induced A549 cell damage

作　　者：李淑芬[1] 宋卓慧[1] 李婷[1] 常永丽[1] 刘燕[1] 赵丽芬 LI Shu-fen;SONG Zhuo-hui;LI Ting;CHANG Yong-li;LIU Yan;ZHAO Li-fen(Department of Physiology,Changzhi Medical College,Changzhi 046000,Shanxi Province,China;Department of Respiratory and Critical Care Medicine,Shanxi Academy of Medical Sciences,Bethune Hospital,Taiyuan 030032,Shanxi Province^China)

机构地区：[1]长治医学院生理学教研室,山西长治046000 [2]山西白求恩医院山西医学科学院呼吸与危重症医学科,山西太原030032

出　　处：《中国临床药理学杂志》2021年第23期3274-3278,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81902020);山西省应用基础研究计划基金资助项目(201901D211469);山西省应用基础研究计划基金资助项目(201801D221444);山西省卫生健康委科研课题基金资助项目(2018126);山西省高等学校科技创新基金资助项目(2019L0662)。

摘　　要：目的探究Hirsutanol A减轻脂多糖(LPS)诱导的A549细胞损伤的作用及机制。方法实验设置对照组(C组)、LPS组(L组)、LPS+miR-17-5p组(L+M组)、LPS+miR-con组(L+M;组)、LPS+miR-17-5p+Hirsutanol A组(L+M+HA组)。用细胞染色计数(CCK-8)法对细胞存活率进行检测,流式细胞术对细胞凋亡率及细胞内活性氧(ROS)水平进行检测,用蛋白质印迹(Western Blot)法检测凋亡蛋白表达水平。结果C组、L组、L+M;组、L+M组及L+M+HA组的细胞存活率分别为(99.80±1.66)%,(81.41±3.05)%,(77.95±3.38)%,(60.98±1.29)%及(87.08±2.97)%,细胞凋亡率分别为(2.91±0.66)%,(12.73±0.91)%,(15.83±0.38)%,(27.84±2.67)%和(7.31±0.75)%,ROS相对含量分别为1.06±0.03,1.75±0.16,2.13±0.12,3.14±0.22,1.21±0.07,差异均有统计学意义(均P<0.05)。L+M+HA组细胞内B淋巴细胞瘤-2(Bcl-2)蛋白表达上升;叉头框蛋白A1(FOXA1)、Bcl-2相关X蛋白(Bax)、胱天蛋白酶-3(caspase-3)的蛋白表达下降。结论Hirsutanol A通过靶向调控miR-17-5p/FOXA1轴促进细胞的存活,抑制细胞发生线粒体依赖性凋亡及ROS积累,减轻LPS诱导的A549细胞损伤。Objective To explore the effect of Hirsutanol A on reducing the A549 cell damage induced by lipopolysaccharide(LPS).Methods We set up control group(C group),LPS group(L group),LPS+miR-17-5p group(L+M group),LPS+miR-con group(L+M;group),LPS+miR-17-5p+Hirsutanol A group(L+M+HA group).Cell counting kit(CCK-8)assey was used to detect cell viability,flow cytometry was used to detect apoptosis rate and intracellular reactive oxygen species(ROS)levels,and Western Blot was used to detect the expression of apoptotic proteins.Results The cell survival rates of the C group,L group,L+M;group,L+M group and L+M+HA group were(99.80±1.66)%,(81.41±3.05)%,(77.95±3.38)%,(60.98±1.29)%,and(87.08±2.97)%;the cell apoptosis rates were(2.91±0.66)%,(12.73±0.91)%,(15.83±0.38)%,(27.84±2.67)%and(7.31±0.75)%;the relative content of ROS were 1.06±0.03,1.75±0.16,2.13±0.12,3.14±0.22,1.21±0.07.The differences were all statistically significant(all P<0.05).In addition,the expression of B-cell lymphoma-2(Bcl-2)in L+M+HA group increased^(-1)the expression of forkhead box protein A1(FOXA1),Bcl-2-associated X protein(Bax),caspase-3 decreased after treated with Hirsutanol A.Conclusion Hirsutanol A promotes cell survival by targeting miR-17-5p/FOXA1 axis,inhibits mitochondrial-dependent apoptosis and ROS accumulation in A549 cells,and reduces the cell damage induced by LPS.

关 键 词：叉头框蛋白A1 A549细胞 活性氧 凋亡 

分 类 号：R97[医药卫生—药品]