响应面法优化三颗针多糖提取工艺  

Optimization of Extraction Process of Polysaccharide from Barberry Root by Response Surface Methodology

作　　者：孙俊乐 顾相瑞 李梦瑶 丁城沣 许志强 候冉冉 Sun Junle;Gu Xiangrui;Li Mengyao;Ding Chengfeng;Xu Zhiqiang;Hou Ranran(College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Shandong,266109)

机构地区：[1]青岛农业大学化学与药学院,山东266109

出　　处：《当代化工研究》2021年第23期128-132,共5页Modern Chemical Research

基　　金：国家自然科学基金青年基金(31802229);青岛农业大学高层次基金(6631121044)。

摘　　要：采取响应面法优化三颗针多糖的水提醇沉提取工艺条件。首先通过研究料液比、提取温度、提取时间3个提取因素对三颗针多糖得率效果的影响,在单因素试验筛选的前提下,响应面法设计、试验与分析,优化了三颗针多糖的提取工艺。本试验优化筛选出三颗针多糖最佳提取条件为液料比20mL/g,提取时间100min,提取温度100℃。在该提取条件下三颗针多糖得率(0.476±0.022)%,相对偏差较小,提取条件稳定可靠,为三颗针多糖的有效提取提供理论依据,为三颗针多糖的活性研究奠定基础。The response surface methodology was used to optimize the water extraction and alcohol precipitation process conditions of the Barberry Root polysaccharide.Firstly,the influence of three factors of material-to-liquid ratio,extraction temperature and extraction time on the yield of Barberry Root polysaccharides was studied.On the basis of single factor test screening,response surface method was used to design and analyze,and then the extraction of Barberry Root polysaccharides was optimized.In this experiment,the optimal extraction conditions of the Barberry Root polysaccharides were as follows,the solid-liquid ratio was 20 mL/g,the extraction time was 100 min,and the extraction temperature was 100℃.Under the extraction conditions,the Barberry Root polysaccharide yield is(0.476±0.022)%which has low relative deviation.The extraction conditions are stable and reliable,which provide a theoretical basis for the effective extraction of the Barberry Root polysaccharide and lay a foundation for the study of the activity of the Barberry Root polysaccharide.

关 键 词：三颗针 多糖 单因素试验 响应面分析法 

分 类 号：S859.3[农业科学—临床兽医学]