高效絮凝菌的分离鉴定及其絮凝条件的优化  

Isolation and Identification of High-efficiency Flocculating Bacteria and Optimization of Flocculation Conditions

作　　者：林映津 陈倩 曾小妹 谢贻冬 付洋 LIN Ying-jin;CHEN Qian;ZENG Xiao-mei(Huachuan Technology Co.,Ltd.,Fuzhou,Fujian 350008;Fujian Engineering and Research Center of Ecological Restoration of Lake,Reseavoir&River Basin,Fuzhou,Fujian 350008)

机构地区：[1]华川技术有限公司,福建福州350008 [2]福建省湖库流域生态修复工程研究中心,福建福州350008

出　　处：《安徽农业科学》2021年第24期6-10,23,共6页Journal of Anhui Agricultural Sciences

基　　金：福州市科技计划项目(2019-S-110)。

摘　　要：采用微生物分离纯化技术从底泥样品中筛选到一株絮凝菌,通过目标菌株的形态学特征和16S rRNA序列分析以鉴定其种属,并对其发酵条件和絮凝条件进行优化。结果表明,XN1-4为黏质沙雷氏菌;XN1-4在发酵28 h后其发酵液的絮凝效果最好。菌株XN1-4的最佳培养条件为培养基初始pH 5.0、培养温度25℃、摇床转速150 r/min;最佳絮凝条件为发酵液投加量4%、助凝剂投加量4%。在最佳絮凝条件下,XN1-4的絮凝率达到98%,且絮凝活性成分主要存在于菌体本身。Using microbial separation and purification technology,a flocculating bacteria was isolated from the sediment samples.The bacterial species were identified characterized based on morphological characteristics and 16S rRNA sequence analysis,and its fermentation and flocculation conditions were optimized.The results showed that the flocculating bacteria XN1-4 belonged to Serratia marcescens;strain XN1-4 had the best flocculation effect in the fermentation broth after cultured for 28 h.The optimal culture conditions of strain XN1-4 were the initial pH of the medium 5.0,the culture temperature of 25℃,and the rotating speed of the shaker 150 r/min;the optimal flocculation conditions were the fermentation broth dosage of 4%and the coagulant dosage of 4%.Under the optimal flocculation conditions,the flocculation rate of XN1-4 reached 98%,and the flocculating active components mainly existed in the bacteria itself.

关 键 词：絮凝菌 分离鉴定 培养条件 絮凝条件 优化 

分 类 号：X52[环境科学与工程—环境工程]