玉米ARF家族基因在雌花序发育过程中的表达分析  

Expression Analysis of ARF Family Genes in Female Inflorescence Development of Maize

作　　者：王景超 程彬 于晓菲 齐云 于军 张君 张娟 WANG Jing-chao;CHENG Bin;YU Xiao-fei(Shandong Product Quality Inspection Research Institute,Jinan,Shandong 250100)

机构地区：[1]山东省产品质量检验研究院,山东济南250100

出　　处：《安徽农业科学》2021年第24期132-135,共4页Journal of Anhui Agricultural Sciences

摘　　要：利用实时定量PCR方法对玉米中的35个ARF家族基因在雌花序不同发育时期的表达模式进行了分析。利用Genevestigator网站中转录组数据库的信息,分析发现,玉米7个不同发育时期均有1到多个ARF基因表达,表明ARF家族基因在玉米不同发育时期的调控中发挥重要作用。转录组数据还显示,每个ARF基因均在玉米花序发育时期具有较高表达水平。为了探究玉米雌花序发育过程中ARF基因家族的表达模式,利用qRT-PCR技术对玉米ARF家族成员在雌花序不同发育时期的基因表达水平进行了检测。根据ARF基因的表达模式,可将这35个基因分成4类:第1类的15个ARF基因在小穗对原基(SPM)时期高表达;第2类的13个ARF基因在小穗原基(SM1)时期高表达;第3类的6个ARF基因在花器官原基(F)时期高表达;第4类的1个ARF基因在花原基(FM)时期高表达。研究结果表明,玉米ARF家族基因在雌花序不同发育时期发挥重要作用。该研究将为解析玉米ARF家族基因在雌花序生长发育过程中的生物学功能提供重要信息。In this study,we analyzed the expression patterns of 35 members of this gene family in female inflorescences at different developmental stages.Using the information of the transcriptome database in Genevestigator website,it was found that one or more ARF genes were expressed in maize at seven different developmental stages,indicating that ARF family genes regulate different developmental processes cooperatively.In addition,each ARF gene was expressed at a high level during inflorescence development.In order to explore the role of ARF family members in the development of female inflorescences of maize,we used real-time quantitative PCR to detect gene expression levels in six female inflorescences at different developmental stages.According to the expression pattern of ARF gene,the 35 ARF genes could be divided into four classes:the first class,15 ARF genes were highly expressed in spikelet-pair meristem(SPM);in the second class,13 ARF genes were highly expressed in spikelet meristem(SM1).Six ARF genes of the third class were highly expressed in flower organ meristem(F)stage,and one ARF gene of the fourth class was highly exprossed in floral primorclium(FM)stage.These results indicated that ARF gene plays an important role in the development of female inflorescence in maize.The results of this study will provide important information for studying the function of ARF gene in the development of female inflorescence in maize.

关 键 词：ARF 雌花序 表达 玉米 

分 类 号：S513[农业科学—作物学]