全身暴露动态吸入一氧化二氮对清醒大鼠电生理指标的影响  

Effects of whole body dynamic inhalation exposure to nitrous oxide on electrophysiological indexes in conscious rats

作　　者：张彦瑾 刘宇轩 单煜恒 聂志勇 王永安 ZHANG Yan-jin;LIU Yu-xuan;SHAN Yu-heng;NIE Zhi-yong;WANG Yong-an(State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;Department of Neurology,the First Medical Centre,Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]军事科学院军事医学研究院毒物药物研究所,抗毒药物与毒理学国家重点实验室,北京100850 [2]解放军总医院第一医学中心神经内科,北京100853

出　　处：《中国药理学与毒理学杂志》2021年第12期929-936,共8页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的建立大鼠全身暴露动态吸入一氧化二氮(N_(2)O)模型与电生理遥测联用系统,探讨吸入N_(2)O对清醒大鼠电生理指标的影响。方法基于空气动力学原理,采用可被电生理遥测信号穿透的有机玻璃建立大鼠全身暴露动态吸入N_(2)O装置;雄性SD大鼠分为空气对照组及30%,50%和70%N_(2)O组,利用生理信号无线遥测仪连续采集吸入前后清醒大鼠的血压、体温、心电(心率、R波、P波、T波、QT间期、QRS间期和PR间期)和脑电(δ波、θ波、α波、β波和γ波)参数变化以及波形图变化。结果与空气对照组相比,50%和70%N_(2)O组大鼠心率、R波、P波、T波和血压均显著增加(P<0.05,P<0.01),QT间期和体温显著下降(P<0.05,P<0.01)。与空气对照组相比,各剂量组δ波(0.5~4 Hz)未出现显著变化;50%和70%N_(2)O组θ波(4~8 Hz)显著增加(P<0.05,P<0.01);70%N_(2)O组α波(8~12 Hz)和γ波(30~48 Hz)均显著下降(P<0.05,P<0.01);50%和70%N_(2)O组β波(12~30 Hz)显著下降(P<0.05,P<0.01)。结论成功建立全身暴露动态吸入N_(2)O模型与电生理遥测联用系统,实现了对大鼠吸入N_(2)O过程中电生理指标的实时采集与变化趋势分析。全身暴露动态吸入N_(2)O可引起大鼠心率加快、血压升高、体温下降,脑电频段中θ波升高,α、β、γ波下降。OBJECTIVE To establish a model of dynamic inhalation of nitrous oxide(N_(2)O) in rats with whole body exposure and an electrophysiological telemetry combined system to explore the effects of N_(2)O inhalation on electrophysiological indicators of awake rats.METHODS In order to establish a device for whole-body dynamic inhalation of nitrous oxide for rats,the principles of aerodynamics were taken into consideration and organic glass that could be transmitted by electrophysiological telemetry signals was selected.Four experiment groups of male SD rats were designed as air control group,30%N_(2)O,50% N_(2)O and 70% N_(2)O groups.The physiological signal telemetry system was used to continuously monitor blood pressure,boby temperature,electrocardiograms(heart rate,R waves,P waves,T waves,QT interval,QRS interval and PR interval) and electroencephalograms(δ waves,θ waves,αwaves,β waves and γ waves) and waveform changes of conscious rats before,during and after exposure to N_(2)O.RESULTS Compared with the air control group,the heart rate、R waves、P waves、T waves and blood pressure increased significantly(P<0.05,P<0.01) in 50% N_(2)O and 70% N_(2)O groups,while QT interval and the body temperature decreased significantly(P<0.05,P<0.01).As for EEG,compared with the air control group,there was no significant δ wave(0.5-4 Hz) change in any of the exposure groups.θ waves(4-8 Hz) were increased in 50% N_(2)O and 70% N_(2)O groups,and the difference was statistically significant(P<0.05,P<0.01).α waves(8-12 Hz) and γ waves(30-48 Hz) were decreased significantly in 70% N_(2)O group(P<0.05,P<0.01).β waves(12-30 Hz) were decreased significantly in50% N_(2)O and 70% N_(2)O groups(P<0.05,P<0.01).CONCLUSION A system for studying the N_(2)O with whole-body dynamic inhalation in rats combined with electrophysiological telemetry has been established,which is capable of the real-time collection and analysis of changes of electrophysiological indexes during the inhalation of nitrous oxide in rats.Whole-boby dynamic inhalat

关 键 词：一氧化二氮 动态吸入 电生理 认知障碍 

分 类 号：R971.2[医药卫生—药品] R99[医药卫生—药学]