SSN6-TUP1复合物在真菌基因转录中的功能和机制  

Function and Mechanism of the SSN6-TUP1 Complex in Fungal Gene Transcription

作　　者：王磊 陆昕 解军 WANG Lei;LU Xin;XIE Jun(Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学基础医学院生物化学与分子生物学教研室,太原030001

出　　处：《中国生物化学与分子生物学报》2021年第12期1561-1568,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：山西省面上青年基金(No.201901D211315);山西省博士科研启动基金(No.SD1906);山西医科大学博士科研启动基金(No.XD1906)资助。

摘　　要：精准的转录调控是保证细胞基因适时适量表达以应对外界环境变化的重要前提。SSN6-TUP1是酵母菌中第一个被鉴定的转录抑制复合物。过去十几年里,在其他的真菌中也发现了功能保守的同源蛋白质复合物。尽管在高等真核生物中未发现SSN6-TUP1的同源蛋白质,但是却鉴定出类似结构和功能的蛋白质复合物。研究发现,SSN6-TUP1复合物参与真菌多种生理过程,它主要是在转录水平通过调控基因的表达实现的。SSN6-TUP1不仅可以参与基因的转录抑制,还能介导基因的转录激活。此外,SSN6-TUP1调控目标基因转录的分子机制也呈现出多样性。本文综述了SSN6-TUP1复合物的蛋白质分子结构、细胞定位、翻译后修饰及其调控基因转录的分子机制,有助于强化人们对SSN6-TUP1参与真菌基因表达调控的理解和认识,也可为研究高等真核生物中类似蛋白质因子的生物学功能提供借鉴。Accurate transcriptional regulation is an important prerequisite to ensure the timely and appropriate expression of cellular genes in response to environmental changes.The SSN6-TUP1 complex is the first identified complex that mediates transcriptional repression in yeast.During the last few decades,conserved homologous proteins of the SSN6-TUP1 complex have been found in other fungi.Although homologous protein of SSN6-TUP1 has not been identified in higher eukaryotes,the protein complex with similar structure and function has been found.Studies have shown that the SSN6-TUP1 complex is involved in a variety of physiological processes in fungi,which is mainly observed at the transcription level through the regulation of gene expression.SSN6-TUP1 not only participates in gene transcriptional repression,but can also mediate gene transcriptional activation.Moreover,SSN6-TUP1 shows diverse molecular mechanisms for regulating targeted gene transcription.In this review,we provide a summary of SSN6-TUP1 molecular structure,cellular localization,post-translational modification and molecular mechanism for mediating gene transcription.This review will strengthen the understanding about the role of SSN6-TUP1 during the regulation of fungal gene expression.Moreover,it will also provide a foundation for the studies on the biological functions of similar protein factors from higher eukaryotes.

关 键 词：SSN6-TUP1 蛋白质结构 细胞定位 翻译后修饰 转录抑制/激活 

分 类 号：Q7[生物学—分子生物学] Q93