刺五加提取物对酒精戒断大鼠海马去甲肾上腺素的影响  

Acanthopanax for alcohol withdrawal in the rat hippocampus norepinephrine

作　　者：王加苇 吴宜艳[1] 刘欣 WANG Jia-wei(Department of Public Health,Mudanjiang Medical University,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2022年第1期4-6,43,共4页Journal of Mudanjiang Medical University

基　　金：牡丹江医学院研究生创新基金(YJSCX-MY17)。

摘　　要：目的探讨刺五加提取物对大鼠酒精戒断后所产生焦虑症的作用以及对大鼠海马中去甲肾上腺素的影响。方法将60只健康SD大鼠随机分为6组:空白组、模型组、高剂量刺五加组、中剂量刺五加组、低剂量刺五加组和阳性药物组,每组10只。除空白组大鼠腹腔注射生理盐水(15 mL/kg)作为对照外,其他五组腹腔注射20%(w/v)酒精(15 mL/kg),1次/d,持续28 d,制备酒精戒断大鼠模型。造模成功后,空白组和模型组用生理盐水(15 mL/kg)灌胃作为对照;高剂量刺五加组、中剂量刺五加组和低剂量刺五加组分别按照900 mg/kg、300 mg/kg、100 mg/kg用温水溶解的刺五加浸膏灌胃;阳性药物组用5 mg/kg的地西泮灌胃。连续给药5 d。高架十字迷宫判断大鼠焦虑情况,HPLC测定大鼠海马中去甲肾上腺素。结果与模型组相比,高剂量刺五加组能够提高大鼠进入开放臂的次数百分比(28.5±6.4)%和停留在开放臂的时间百分比(20.4±4.8)%(P<0.001);HPLC结果显示,高剂量刺五加浸膏组海马中去甲肾上腺素的浓度为(269.7±89.9)μg/g,相对于模型组显著降低(P<0.05),与阳性药物组无统计学差异(P>0.05);中剂量刺五加组、低剂量刺五加组与模型组相比无统计学差异(P>0.05)。结论刺五加提取物可减轻酒精戒断大鼠的焦虑样行为,其机制可能是通过降低海马中的去甲肾上腺素水平实现的。Objective To explore the effect of Acanthopanax senticosus extract on the anxiety caused by alcohol withdrawal in rats and the effect on norepinephrine in the hippocampus of rats.Methods Sixty healthy SD rats were randomly divided into 6 groups:blank group,model group,high,medium and low dose Acanthopanax senticosus group and positive drug group.10 per group.During the modeling period,rats in the blank group were intraperitoneally injected with normal saline(15 mL/kg)as a control;the other five groups were intraperitoneally injected with 20%(w/v)alcohol(15 mL/kg)for 28 days,and the injection was stopped for 3 days.Rat model of alcohol withdrawal.During the medication period,the blank group and the model group were given normal saline(15 mL/kg)as a control;the high,medium and low Acanthopanax senticosus groups were dissolved with warm water at 900 mg/kg,300 mg/kg,and 100 mg/kg,respectively.Gavage after the extract;the positive drug group was given 5 mg/kg diazepam by gavage.Administration for 5d.The elevated plus maze was used to judge the anxiety of the rats,and the norepinephrine in the hippocampus of the rats was determined by HPLC.Results(1)Behavioral evaluation showed that compared with the model group,the high-dose Acanthopanax senticosus group can increase the percentage of times the rats enter the open arm(28.5±6.4)%(P=0.0056)and the percentage of time spent in the open arm(20.4±4.8)%,statistically significant(P=0.0088);(2)HPLC results showed that the concentration of norepinephrine in the hippocampus of the high-dose Acanthopanax senticosus extract group was(269.7±89.90)μg/g,Significantly lower than the model group(P=0.0328).Conclusion Acanthopanaxsenticosus extract can reduce anxiety-like behaviors in alcohol withdrawal rats,andit may achieve its mechanism by reducing the level of norepinephrine in the hippocampus.

关 键 词：刺五加 酒精使用障碍 去甲肾上腺素 

分 类 号：R247.1[医药卫生—中医临床基础]