增生性糖尿病视网膜病变患者房水及血清IL-1β与IL-1Ra含量分析  

Levels of IL-1βand IL-1Ra in aqueous humor and serum of patients with proliferative diabetic retinopathy

作　　者：林仲 牛彤彤[2] 杜林 张晨曦 张绍丹 王海林[2] Lin Zhong;Niu Tongtong;Du lin;Zhang Chenxi;Zhang Shaodan;Wang Hailin(The Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical University,Wenzhou 325027,China;Department of Ophthalmology,The Fourth People’s Hospital of Shenyang,Key Laboratory of Eye Diseases of Shenyang,Shenyang 110031,China)

机构地区：[1]温州医科大学附属眼视光医院,325027 [2]沈阳市第四人民医院眼科,沈阳市眼病中心重点实验室,110031

出　　处：《眼科》2021年第6期449-452,共4页Ophthalmology in China

基　　金：沈阳市重点实验室建设项目(F15-160-1-00);沈阳市重点科技研发计划项目(17-230-9-03);温州市基础性医疗卫生科技项目(Y2020364)。

摘　　要：目的探索增生性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者房水及血清白介素1β(IL-1β)、白介素1受体拮抗剂(IL-1Ra)与血管内皮生长因子(VEGF)水平的变化及其意义。设计前瞻性比较性病例系列。研究对象36例PDR患者与26例非糖尿病白内障患者(对照组)。方法采集患者外周血,并于白内障摘除术或玻璃体切除术中抽取未稀释的房水。采用免疫磁珠多重因子检测方法分析房水与血清中IL-1β、IL-1Ra、VEGF含量。比较两组细胞因子含量的差异。主要指标房水与血清中IL-1β、IL-1Ra、VEGF含量。结果PDR组房水中的IL-1β(6.7±4.3 pg/ml)和IL-1Ra(657.9±445.6 pg/ml)含量均高于对照组(3.3±3.2 pg/ml和300.8±368.0 pg/ml,P均=0.001);血清中的IL-1β(7.6±4.6 pg/ml)和IL-1Ra(437.8±270.2 pg/ml)含量亦高于对照组(4.9±3.7 pg/ml和279.2±226.7 pg/ml,P均=0.02)。两组房水及血清中VEGF含量差异均无统计学意义。两组房水或血清中IL-1β含量均与各自同一样本中IL-1Ra的含量呈高度正相关。PDR组房水与血清中的IL-1β(r=0.50,P=0.003)、房水与血清中的IL-1Ra(r=0.66,P<0.001)含量均为正相关,但房水与血清中的VEGF含量之间无明显相关(r=-0.06,P=0.72)。结论PDR患者房水与血清中炎症因子升高的同时伴随抗炎因子的代偿性增高。针对炎症反应的调控可能为DR的治疗开拓新的思路。血清中炎症因子与抗炎因子水平可能作为DR发生的生物标志物。Objective To explore the level changes of interleukin-1β(IL-1β),interleukin-1 receptor antagonist(IL-1Ra),and vascular endothelial growth factor(VEGF)in aqueous humor and serum of patients with proliferative diabetic retinopathy(PDR).Design Prospective comparative case seiries.Participants 36 PDR patients and 26 cataract patients without diabetes(control group)were enrolled.Methods The peripheral blood and undiluted aqueous humor(operatively)of all patients were collected.The cytokine concentrations of aqueous humor and serum were analyzed by immune multiplex bead-based assay.The general information and cytokine concentration were compared between the two groups.The correlation analyses were also performed for the cytokines concentration of aqueous humor and serum.Main Outcome Measures The concentrations of IL-1β,IL-1Ra,VEGF in aqueous humor and serum.Results Both the concentration of IL-1β(6.7±4.3 pg/ml)and IL-1Ra(657.9±445.6 pg/ml)of aqueous humor in PDR group were higher than that in control group(3.3±3.2 pg/ml and 300.8±368.0 pg/ml,both P=0.001).The concentration of IL-1β(7.6±4.6 pg/ml)and IL-1Ra(437.8±270.2 pg/ml)of serum in PDR group were also higher than that in control group(4.9±3.7 pg/ml and 279.2±226.7 pg/ml,both P=0.02).There was no significant difference for the concentration of VEGF of aqueous humor and serum between the two groups.The concentration of IL-1βof aqueous humor or serum was highly and positively correlated with the concentration of IL-1Ra of the same sample.The concentration of IL-1β(r=0.50,P=0.003)and IL-1Ra(r=0.66,P<0.001)of aqueous humor was positively correlated with that of serum,respectively,in PDR group.However,there was no correlation for concentration of VEGF of aqueous humor and serum(r=-0.06,P=0.72).Conclusion The anti-inflammation cytokine compensatively increases as the inflammation cytokine increases in both aqueous humor and serum in patients with PDR.The regulation of this inflammation response may represent a novel modality in the treatment of diabetic ret

关 键 词：增生性糖尿病视网膜病变 VEGF IL-1Β IL-1RA 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]