反硝化颗粒污泥培养及工艺快速启动  

Cultivation and Fast Start-up of Denitrifying Granular Sludge

作　　者：王朝朝 马骏 殷春雨 武新娟 朱书浩 李思敏 WANG Zhao-zhao;MA Jun;YIN Chun-yu;WU Xin-juan;ZHU Shu-hao;LI Si-min(Hebei Technology Innovation Center of Water Pollution Control and Water Ecological Remediation,Handan 056038,China;Handan Key Laboratory of Water Utilization Technology,Handan 056038,China;College of Energy and Environmental Engineering,Hebei University of Engineering,Handan 056038,China)

机构地区：[1]河北省水污染控制与水生态修复技术创新中心,河北邯郸056038 [2]邯郸市水利用技术重点实验室,河北邯郸056038 [3]河北工程大学能源与环境工程学院,河北邯郸056038

出　　处：《中国给水排水》2021年第23期1-8,共8页China Water & Wastewater

基　　金：河北省高校青年拔尖人才计划项目(BJ2019029);河北省自然科学基金资助项目(E2021402011)。

摘　　要：在UASB反应器中接种絮状活性污泥,通过调节进水底物浓度、提高上升流速等方式培养并富集反硝化颗粒污泥。经过45 d的运行快速培养出边缘清晰、白色且粒径均匀的反硝化颗粒污泥。在反硝化颗粒污泥形成后,总氮去除负荷(NRR)及碳去除负荷(CRR)分别达到0.65和1.97 kg/(m^(3)·d),NO_(3)^(-)-N与COD最大比降解速率分别为68.90和182.63 mg/(gMLSS·d),表明反硝化颗粒污泥工艺具有较好的脱氮除碳性能。在反硝化颗粒污泥培养的过程中,ΔCOD/ΔNO_(3)^(-)-N均值为3.35,始终大于理论值2.86。胞外聚合物(EPS)中蛋白质、多糖的含量及比值也逐渐增加,使微生物呈疏水性。三维荧光光谱(3D-EEM)分析表明,色氨酸及酪氨酸等蛋白质类物质在加速反硝化颗粒污泥形成过程中起到重要作用。In the UASB reactor,flocculent activated sludge was inoculated to enhance the enrichment of denitrifying granular sludge by adjusting the substrate concentration and increasing the ascending flow rate.After 45 d operation,denitrifying granular sludge featured with clear edges,milky white and uniform particle size was rapidly cultivated.After the formation of denitrifying granular sludge,the total nitrogen removal load(NRR)and carbon removal load(CRR)reached 0.65 kg/(m^(3)·d)and 1.97 kg/(m^(3)·d),respectively,and the maximum specific degradation rates of NO_(3)^(-)-N and COD were 68.90 mg/(gMLSS·d)and 182.63 mg/(gMLSS·d),respectively,indicating that the denitrifying granular sludge process exhibited a good nitrogen and carbon removal performance.In the process of denitrification granular sludge cultivation,the average value ofΔCOD/ΔNO_(3)^(-)-N ratio was 3.35,which was always higher than the theoretical value of 2.86.The contents of protein(PN)and polysaccharide(PS)in extracellular polymer substance(EPS),and PN/PS ratio also increased gradually,which made the microorganisms to exhibit hydrophobic.At the same time,three-dimensional fluorescence spectrum(3D-EEM)analysis results showed that tryptophan,tyrosine and other proteins played important roles in accelerating the formation of denitrifying granular sludge.

关 键 词：反硝化颗粒污泥 UASB反应器 脱氮性能 理化特性 

分 类 号：TU992[建筑科学—市政工程]