活性污泥系统中S/N对硝酸盐异化还原过程的影响  

Effect of S/N Ratio on Dissimilatory Nitrite Reduction Process in Activated Sludge System

作　　者：王晓婷 李晓玲[1] 芦昭霖 张鹏程 WANG Xiao-ting;LI Xiao-ling;LU Zhao-lin;ZHANG Peng-cheng(Key Laboratory for Water-supply and Drainage,School of Civil Engineering,Chang’an University,Xi’an 710061,China;Xi’an Secondary Water Supply Management Center,Xi’an 710016,China;Southwest Municipal Engineering Design&Research Institute of China,Chengdu 610036,China)

机构地区：[1]长安大学建筑工程学院住房和城乡建设部给水排水重点实验室,陕西西安710061 [2]西安市二次供水管理中心,陕西西安710016 [3]中国市政工程西南设计研究总院有限公司,四川成都610036

出　　处：《中国给水排水》2021年第23期95-100,共6页China Water & Wastewater

基　　金：陕西省自然科学基础研究项目(2019JQ-686);国家自然科学基金青年基金资助项目(51808045)。

摘　　要：硝酸盐异化还原成铵(DNRA)作为硝酸盐异化还原途径的重要过程而备受关注,为研究活性污泥系统中硫对硝酸盐异化还原过程(反硝化和DNRA过程)的影响,以硫化钠为电子供体、硝酸盐为电子受体,在活性污泥系统中分析控制不同S/N的效果。当S/N=1.0时,系统主要进行硫自养反硝化;当S/N分别为1.3、1.5、1.7、2.0时,系统同时发生硫自养反硝化和DNRA过程,且DNRA所占的百分比(P_(DNRA))在S/N=1.7时最大,说明活性污泥硫自养反硝化体系中电子供体充足时会出现DNRA过程,且P_(DNRA)随电子供体/受体的比例不同而发生改变。系统中的氧化还原电位(ORP)对硝酸盐还原过程有一定的指示作用,ORP值越低越有利于DNRA的发生。微生物测定结果表明,Thiobacills、Hydrogenophilaceae、Pseudoxanthomonas和Burkholderiaceae是与硫自养反硝化和DNRA过程相关的主要菌群,进一步确定了DNRA过程的发生。Dissimilatory nitrite reduction to ammonia(DNRA),an important component of nitrate dissimilatory reduction pathway,has attracted much attention.To explore the effect of sulfur on denitrification and DNRA process in activated sludge system,effect of different S/N ratio on nitrate dissimilation reduction pathway in activated sludge system was analyzed by using sodium sulfide as electron donor and nitrate as electron acceptor.When S/N ratio was 1.0,sulfur autotrophic denitrification process was dominated in the system.When S/N ratios were 1.3,1.5,1.7 and 2.0,respectively,both sulfur autotrophic denitrification and DNRA processes occurred simultaneously in the system,and the proportion of DNRA(P_(DNRA))reached the maximum when S/N ratio was 1.7.The results indicated that DNRA occurred when the electron donor was sufficient in the activated sludge sulfur autotrophic denitrification system,and the P_(DNRA) changed with the ratio of electron donor to acceptor.Oxidation-reduction potential(ORP)of the system could indicate the nitrate reduction process to some extent,and lower ORP was more beneficial to DNRA occurrence.Thiobacills,Hydrogenophilaceae,Pseudoxanthomonas and Burkholderiaceae were main microbial community of the process of sulfur autotrophic denitrification and DNRA,further confirming the occurrence of DNRA process.

关 键 词：硫自养反硝化 S/N 硝酸盐异化还原成铵 氧化还原电位 菌群分析 

分 类 号：TU992[建筑科学—市政工程]