Establishment of an oligoasthenospermia mouse model based on TAp73 gene suppression  

作　　者：Hong-Juan Liu Meng-Yun Deng Yan-Yan Zhu De-Ling Wu Xiao-Hui Tong Li Li Lei Wang Fei Xu Tong-Sheng Wang 

机构地区：[1]School of Integrated Traditional Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei,China [2]Anhui Province Key Laboratory of Chinese Medical Formula,School of Pharmacy,Anhui University of Chinese Medicine,Hefei,China

出　　处：《Animal Models and Experimental Medicine》2021年第4期351-358,共8页动物模型与实验医学（英文）

基　　金：This project was supported by the Natural Science Foundation of Anhui Provincial Department of Education(no.KJ2020A0386);National Natural Science Foundation of China(no.82174162).

摘　　要：Background:Oligoasthenospermia is one of the main causes of male infertility.Researchers usually use chemical drugs to directly damage germ cells to prepare oligoasthenospermia models,which disregards the adhesion and migration between spermatogenic cells and Sertoli cells.TAp73 is a critical regulator of the adhesin of germ cell;thus,we sought to explore a novel oligoasthenospermia model based on TAp73 gene suppression.Methods:Mice in the Pifithrin-αgroup were injected intraperitoneally with 2.5 mg/kg Pifithrin-α(TAp73 inhibitor)daily for 30 consecutive days.Reproductive hormone levels and epididymal sperm quality,as well as the network morphology of Sertoli cells were tested.Results:Sperm density,motility,and the relative protein and mRNA expression of TAp73 and Nectin 2 were obviously decreased in the Pifithrin-αgroup compared with the normal control group.No significant distinction was observed in the relative mRNA and protein expression of ZO-1.Furthermore,the tight junctions(TJs)and api-cal ectoplasmic specialization(ES)were destroyed in the Pifithrin-αgroup.Conclusion:The above results indicate that we successfully established a new oli-goasthenospermia mouse model.This study provides a foundation for further explo-ration of the roles of TAp73 genes during spermatogenesis and provides new research objects for further oligospermia research and future drug discovery.

关 键 词：ectoplasmic specialization Nectin 2 OLIGOASTHENOSPERMIA TAp73 ZO-1 

分 类 号：R698.2[医药卫生—泌尿科学] R-332[医药卫生—外科学]