In-depth examination of hyperproliferative healing in two breeds of Sus scrofa domesticus commonly used for research  

作　　者：Colton H.Funkhouser Liam D.Kirkpatrick Robert D.Smith Lauren T.Moffatt Jeffrey W.Shupp Bonnie C.Carney 

机构地区：[1]Firefighters'Burn and Surgical Research Laboratory,MedStar Health Research Institute,Washington,District of Columbia,USA [2]Department of Biochemistry and Molecular and Cellular Biology,Georgetown University Medical Center,Washington,District of Columbia,USA [3]Department of Surgery,Georgetown University School of Medicine,Washington,District of Columbia,USA [4]The Burn Center,Department of Surgery,MedStar Washington Hospital Center,Washington,District of Columbia,USA

出　　处：《Animal Models and Experimental Medicine》2021年第4期406-417,共12页动物模型与实验医学（英文）

基　　金：This work was funded in part by R01 AR070851-01A1.

摘　　要：Background:Wound healing can result in various outcomes,including hypertrophic scar(HTS).Pigs serve as models to study wound healing as their skin shares physi-ologic similarity with humans.Yorkshire(Yk)and Duroc(Dc)pigs have been used to mimic normal and abnormal wound healing,respectively.The reason behind this differential healing phenotype was explored here.Methods:Excisional wounds were made on Dc and Yk pigs and were sampled and imaged for 98 days.PCR arrays were used to determine differential gene expression.Vancouver Scar Scale(VSS)scores were given.Re-epithelialization was analyzed.H&E,Mason's trichrome,and immunostains were used to determine cellularity,col-lagen content,and blood vessel density,respectively.Results:Yk wounds heal to a“port wine”HTS,resembling scarring in Fitzpatrick skin types(FST)I-III.Dc wounds heal to a dyspigmented,non-pliable HTS,resembling scarring in FST IV-VI.Gene expression during wound healing was differentially regulated versus uninjured skin in 40/80 genes,15 of which differed between breeds.Yk scars had a higher VSS score at all time points.Yk and Dc wounds had equivalent re-epithelialization,collagen disorganization,and blood vessel density.Conclusions:Our findings demonstrate that Dc and Yk pigs can produce HTS.Wound creation and healing were consistent among breeds,and differences in gene expression were not sufficient to explain differences in resulting scar phenotype.Both pig breeds should be used in animal models to investigate novel therapeutics to provide insight into a treatment's effectiveness on various skin types.

关 键 词：animal models BURNS Fitzpatrick skin type hypertrophic scar wound healing 

分 类 号：R644[医药卫生—外科学] R-332[医药卫生—临床医学]