Evaluation of bioactive flavonoids in Citri Reticulatae Pericarpium from different regions and its association with antioxidant andα-glucosidase inhibitory activities  

作　　者：Na Liu Jia-Chen Sun Wen-Na Yang Di Liang Lan-Ping Guo Xia Li Wen-Yuan Gao 

机构地区：[1]Tianjin Key Laboratory for Modern Drug Delivery&High-Efficiency,School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300193,China [2]School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China [3]National Resource Center for Chinese Materia Medica,Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Traditional Medicine Research》2022年第1期1-9,共9页TMR传统医学研究

基　　金：supported by the National Natural Science Foundation of China(No.81872956);National Key R&D Program of China(No.2019YFC1710604);Key Project at Central Government Level(No.2060302);the Science and Technology Project of Tianjin(No.18ZXXYSY00080).

摘　　要：Background:To explore the differences of 14 batches of Citri Reticulatae Pericarpium from different regions.Methods:The main aim of this study was to develop a high-performance liquid chromatography method-photodiode array detection for determining the contents of five flavonoids and the chromatographic fingerprints of Citri Reticulatae Pericarpium from different regions.Theα-glucosidase inhibitory activities and antioxidant properties of Citri Reticulatae Pericarpium,based on free-radical scavenging assays against 1,1-diphenyl-2-picrylhydrazyl,2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),and hydroxyl radicals,were estimated and compared.Results:Among the tested compounds,the content of hesperidin(13.386-68.235 mg/g)was the highest and that of hesperitin(0.045-0.277 mg/g)was the lowest.In comparing different sources of Citri Reticulatae Pericarpium,the contents of narirutin in Citri Reticulatae Pericarpium from Guangdong(0.824-0.851 mg/g)and Sichuan(1.069-1.204 mg/g)provinces of China were lower than in other provinces.In contrast,nobiletin(8.429-12.237 mg/g)and tangeretin(3.947-4.613 mg/g)were most abundant in Guangdong sources of Citri Reticulatae Pericarpium,followed by samples from Sichuan Province(nobiletin:6.761-7.658 mg/g;tangeretin:3.422-3.933 mg/g).Correlation analysis showed that nobiletin and tangeretin were the main contributors to the antioxidant capacity,and narirutin was the main active component inhibiting theα-glucosidase activity of Citri Reticulatae Pericarpium.Conclusion:This work revealed that the intrinsic quality of Citri Reticulatae Pericarpium was affected by different growing regions,which provides a scientific basis for controlling the quality of Citri Reticulatae Pericarpium and rationally developing and utilizing Citri Reticulatae Pericarpium.

关 键 词：Citri Reticulatae Pericarpium FLAVONOIDS HPLC-PDA antioxidant activity α-glucosidase inhibitory activity 

分 类 号：F42[经济管理—产业经济]