脱除SPVD甘薯组培苗快繁及移栽研究  

Study on rapid propagation and transplanting of tissue culture seedlings of sweet potato with SPVD removed

作　　者：袁蕊 唐伟 孙书军[1] 赵路宽 胡杨 马居奎 王洁 曹清河[1] 周志林[1] YUAN Rui;TANG Wei;SUN Shujun;ZHAO Lukuan;HU Yang;MA Jukui;WANG Jie;CAO Qinghe;ZHOU Zhilin(Xuzhou Institute of Agricultural Sciences in Xuhuai District of Jiangsu Province,Xuzhou 221131,China)

机构地区：[1]江苏徐淮地区徐州农业科学研究所,江苏徐州221131

出　　处：《北方农业学报》2021年第5期112-117,共6页Journal of Northern Agriculture

基　　金：国家重点研发计划项目(2019YFD1000701-4);国家甘薯产业技术体系项目(CARS-10-B1-2020)。

摘　　要：【目的】筛选感甘薯病毒病(SPVD)甘薯脱毒组培苗快繁最佳激素组合,为甘薯脱毒种苗在生产上的应用提供技术支撑。【方法】以徐薯22和阜徐薯20为试验材料,通过不同脱毒次数、病毒抑制剂、浓度激素(NAA/6-BA)组合、继代培养时间及炼苗等条件对茎尖分生组织培养,检测不同处理对SPVD的脱除率,测量组培苗株高,统计移栽成活率。【结果】甘薯褪绿矮化病毒(SPCSV)一次茎尖分生组织培养脱除率达100%,甘薯羽状斑驳病毒(SPFMV)二次茎尖分生组织培养脱除率达100%;病毒抑制剂宁南·嘧肽预处理SPFMV茎尖脱除率达100%;徐薯22组培苗快繁最佳激素组合为0.05 mg/L NAA、1.0 mg/L 6-BA,阜徐薯20组培苗快繁最佳激素组合为0.01 mg/L NAA、1.0 mg/L 6-BA;徐薯22和阜徐薯20炼苗处理在继代培养60 d时,最高成活率达86.67%和83.33%。【结论】二次茎尖分生组织培养可完全脱除SPVD,病毒抑制剂宁南·嘧肽预处理可提高SPFMV茎尖脱除率,组培苗快繁的最佳激素组合为0.01~0.05 mg/L NAA、1.0 mg/L 6-BA,炼苗可显著提高组培苗移栽成活率。【Objective】To screen the best hormone combination for rapid propagation of sweet potato virus-free tissue culture seedlings susceptible to sweet potato virus disease(SPVD),and to provide technical support for the application of virus-free sweet potato seedlings in production.【Methods】Using Xushu 22 and Fuxushu 20 as test materials,the shoot apical meristem was cultured with varying detoxification times,virus inhibitors,combination of hormone concentration(NAA/6-BA),subculture time and seedling cultivation.The removal rate of SPVD by different treatments was detected.The plant height of tissue cultured seedlings was measured.The survival rate of transplanting was counted.【Results】The removal rate of sweet potato chlorotic stunt virus(SPCSV)reached 100%in primary shoot apex meristem culture,and the removal rate of sweet potato feathery mottle virus(SPFMV)reached 100%in secondary shoot apex meristem culture.The removal rate of SPFMV stem tip pretreatment with virus inhibitor Ningnan·pyripeptide reached 100%.The optimal hormone combination for fast propagation of Xushu 22 tissue cultured seedlings was 0.05 mg/L NAA,1.0 mg/L 6-BA,and the optimal hormone combination for fast propagation of Fuxushu 20 tissue cultured seedlings was 0.01 mg/L NAA,1.0 mg/L 6-BA.The highest survival rate for Xushu 22 and Fuxushu 20 treated seedlings when subcultured for 60 days was 86.67%and 83.33%.【Conclusion】Secondary shoot apex meristem culture could completely eliminate SPVD.Virus inhibitor Ningnan·pyripeptide pretreatment increased the removal rate of SPFMV shoot apex.The optimal hormone combination for tissue culture seedlings for rapid propagation was 0.01-0.05 mg/L NAA,1.0 mg/L 6-BA.Refining seedlings increased the survival rate of transplanted tissue culture seedlings significantly.

关 键 词：甘薯 甘薯病毒病(SPVD) 茎尖脱毒 组织培养 快繁 移栽 

分 类 号：S531[农业科学—作物学]