银翘解毒颗粒中金银花掺伪检查  

Adulteration Inspection of Lonicerae Japonicae Flos in Yinqiaojiedu Granules

作　　者：覃丽郦 白桂昌 张慧 何颂华 Qin Lili;Bai Guichang;Zhang Hui;He Songhua(Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Institute of Food and Drug Control,NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine,Chinese Material Medica,Nanning 530021,China)

机构地区：[1]广西中医药大学,南宁530200 [2]广西食品药品检验所,国家药品监督管理局中药材质量监测与评价重点实验室,南宁530021

出　　处：《中国药事》2021年第12期1375-1382,共8页Chinese Pharmaceutical Affairs

摘　　要：目的:建立银翘解毒颗粒中金银花掺伪检查方法,考察生产企业是否存在使用山银花代替金银花投料现象。方法:采用高效液相色谱-串联质谱法,色谱柱为Waters CORTECS(150 mm×4.6 mm,2.7μm),以乙腈和水为流动相进行梯度洗脱,流速为0.5 mL·min^(-1)。结果:灰毡毛忍冬皂苷乙在0.0440~5.504μg·mL^(-1)呈良好的线性关系,平均回收率为101.49%,RSD=5.97%(n=9);川续断皂苷乙在0.0458~5.720μg·mL^(-1)呈良好的线性关系,平均回收率为104.71%,RSD=4.99%(n=9)。结论:本方法灵敏、准确,可作为银翘解毒颗粒中金银花掺伪检查方法。Objective:To establish the identification method for the adulteration of Lonicerae Japonicae Flos in Yinqiaojiedu Granules to investigate whether there is a phenomenon of using Lonicerae Flos to replace Lonicerae Japonicae Flos in the manufacture enterprises.Methods:LC-MS method was used with Waters CORTECS (150 mm×4.6 mm,2.7μm) as analytical column,acetonitrile and water were used as mobile phase for gradient elution,the flow rate was 0.5 mL·min^(-1).Results:Macranthoidin B showed good linear relationship in the range of 0.0440-5.504μg·mL^(-1),and the average recovery was 101.49%with RSD=5.97%(n=9).Dipsacoside B showed good linear relationship in the range of 0.0458-5.720μg·mL^(-1),and the average recovery was 104.71%with RSD=4.99%(n=9).Conclusion:The method is sensitive and accurate,which can be applied to adulteration detection of Lonicerae Japonicae Flos in Yinqiaojiedu Granules.

关 键 词：银翘解毒颗粒 金银花 山银花 灰毡毛忍冬皂苷乙 川续断皂苷乙 高效液相色谱-串联质谱法 

分 类 号：R917[医药卫生—药物分析学]