膜荚黄芪CBF基因的克隆及表达分析  

Cloning and expression analysis of CBF gene in Astragalus membranaceus

作　　者：李俊杰 赵洋 哈洋 苏喆莹 王景然 全雪丽[1] 吴松权[1] LI Junjie;ZHAO Yang;HA Yang;SU Zheying;WANG Jingran;QUAN Xueli;WU Songquan(Agricultural College of Yanbian University,Yanji Jilin 133002,China;Taitou Government,Shouguang Shandong 262735,China)

机构地区：[1]延边大学农学院,吉林延吉133000 [2]台头镇人民政府,山东寿光262735

出　　处：《延边大学农学学报》2021年第4期9-16,共8页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金项目(21462044);国家自然科学基金项目(30860036);吉林省自然科学基金项目(YDZJ202101ZYTS197);吉林省自然科学基金项目(201115228)。

摘　　要：为探究膜荚黄芪CBF基因的结构与功能,该试验采用RACE技术克隆得到了膜荚黄芪CBF基因,命名为AmCBF。分析结果表明:AmCBF序列全长cDNA为827 bp,开放阅读框为642 bp,在线分析软件预测其编码213个氨基酸,等电点5.84,分子量24066.86 Da,是亲水性蛋白,定位于细胞质中。实时荧光定量PCR分析结果表明:AmCBF在低温和干旱胁迫时基因表达上调,且在低温处理时表达量较高。To explore the structure and function of CBF(C-repeat binding factor)gene of Astragalus membranaceus,this study cloned CBF gene by using the RACE technology and designated this gene as AmCBF.The results showed that the full length of AmCBF cDNA was 827 bp with 642 bp open reading frame.The analysis of online software indicated that AmCBF encoded 213 amino acids with isoelectric point of 5.84 and molecular weight of 24066.86 Da,which belonged to hydrophilic protein and located in the cytoplasm.The analysis of RealTime PCR indicated that AmCBF expression was upregulated under low temperature and drought stress,and the highest AmCBF expression was at low temperature treatment.

关 键 词：膜荚黄芪 CBF基因 基因克隆 表达分析 

分 类 号：S567.239[农业科学—中草药栽培]